This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1ptv

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:10, 14 February 2024) (edit) (undo)
 
(17 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1ptv.gif|left|200px]]<br /><applet load="1ptv" size="350" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1ptv, resolution 2.3&Aring;" />
 
-
'''CRYSTAL STRUCTURE OF PROTEIN TYROSINE PHOSPHATASE 1B COMPLEXED WITH PHOSPHOTYROSINE'''<br />
 
-
==Overview==
+
==CRYSTAL STRUCTURE OF PROTEIN TYROSINE PHOSPHATASE 1B COMPLEXED WITH PHOSPHOTYROSINE==
-
The crystal structures of a cysteine-215--&gt;serine mutant of protein, tyrosine phosphatase 1B complexed with high-affinity peptide substrates, corresponding to an autophosphorylation site of the epidermal growth, factor receptor were determined. Peptide binding to the protein, phosphatase was accompanied by a conformational change of a surface loop, that created a phosphotyrosine recognition pocket and induced a, catalytically competent form of the enzyme. The phosphotyrosine side chain, is buried within the period and anchors the peptide substrate to its, binding site. Hydrogen bonds between peptide main-chain atoms and the, protein contribute to binding affinity, and specific interactions of, acidic residues of the peptide with basic residues on the surface of the, enzyme confer sequence specificity.
+
<StructureSection load='1ptv' size='340' side='right'caption='[[1ptv]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1ptv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PTV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PTV FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PTR:O-PHOSPHOTYROSINE'>PTR</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ptv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ptv OCA], [https://pdbe.org/1ptv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ptv RCSB], [https://www.ebi.ac.uk/pdbsum/1ptv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ptv ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/PTN1_HUMAN PTN1_HUMAN] Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK; inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion.<ref>PMID:21135139</ref> <ref>PMID:22169477</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pt/1ptv_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ptv ConSurf].
 +
<div style="clear:both"></div>
-
==Disease==
+
==See Also==
-
Known diseases associated with this structure: Abdominal body fat distribution, modifier of OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=176885 176885]], Insulin resistance, susceptibility to OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=176885 176885]]
+
*[[Tyrosine phosphatase 3D structures|Tyrosine phosphatase 3D structures]]
-
 
+
== References ==
-
==About this Structure==
+
<references/>
-
1PTV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=TYR:'>TYR</scene> and <scene name='pdbligand=PHS:'>PHS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PTV OCA].
+
__TOC__
-
 
+
</StructureSection>
-
==Reference==
+
-
Structural basis for phosphotyrosine peptide recognition by protein tyrosine phosphatase 1B., Jia Z, Barford D, Flint AJ, Tonks NK, Science. 1995 Jun 23;268(5218):1754-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7540771 7540771]
+
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Protein-tyrosine-phosphatase]]
+
[[Category: Large Structures]]
-
[[Category: Single protein]]
+
[[Category: Barford D]]
-
[[Category: Barford, D.]]
+
[[Category: Jia Z]]
-
[[Category: Jia, Z.]]
+
-
[[Category: PHS]]
+
-
[[Category: TYR]]
+
-
[[Category: acetylation]]
+
-
[[Category: hydrolase]]
+
-
[[Category: peptide]]
+
-
[[Category: phosphorylation]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:41:32 2008''
+

Current revision

CRYSTAL STRUCTURE OF PROTEIN TYROSINE PHOSPHATASE 1B COMPLEXED WITH PHOSPHOTYROSINE

PDB ID 1ptv

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ptv"
Personal tools