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1q09

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[[Image:1q09.gif|left|200px]]<br /><applet load="1q09" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1q09, resolution 2.50&Aring;" />
 
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'''Crystal structure of the Zn(II) form of E. coli ZntR, a zinc-sensing transcriptional regulator (space group I4122)'''<br />
 
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==Overview==
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==Crystal structure of the Zn(II) form of E. coli ZntR, a zinc-sensing transcriptional regulator (space group I4122)==
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The earliest of a series of copper efflux genes in Escherichia coli are controlled by CueR, a member of the MerR family of transcriptional activators. Thermodynamic calibration of CueR reveals a zeptomolar (10(-21) molar) sensitivity to free Cu+, which is far less than one atom per cell. Atomic details of this extraordinary sensitivity and selectivity for +1transition-metal ions are revealed by comparing the crystal structures of CueR and a Zn2+-sensing homolog, ZntR. An unusual buried metal-receptor site in CueR restricts the metal to a linear, two-coordinate geometry and uses helix-dipole and hydrogen-bonding interactions to enhance metal binding. This binding mode is rare among metalloproteins but well suited for an ultrasensitive genetic switch.
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<StructureSection load='1q09' size='340' side='right'caption='[[1q09]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1q09]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q09 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Q09 FirstGlance]. <br>
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1Q09 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q09 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1q09 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q09 OCA], [https://pdbe.org/1q09 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1q09 RCSB], [https://www.ebi.ac.uk/pdbsum/1q09 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1q09 ProSAT]</span></td></tr>
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Molecular basis of metal-ion selectivity and zeptomolar sensitivity by CueR., Changela A, Chen K, Xue Y, Holschen J, Outten CE, O'Halloran TV, Mondragon A, Science. 2003 Sep 5;301(5638):1383-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12958362 12958362]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ZNTR_ECOLI ZNTR_ECOLI] Zinc-responsive transcriptional regulator of zntA.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/q0/1q09_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1q09 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Changela, A.]]
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[[Category: Changela A]]
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[[Category: Chen, K.]]
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[[Category: Chen K]]
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[[Category: Halloran, T V.O.]]
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[[Category: Holschen J]]
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[[Category: Holschen, J.]]
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[[Category: Mondragon A]]
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[[Category: Mondragon, A.]]
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[[Category: O'Halloran TV]]
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[[Category: Outten, C E.]]
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[[Category: Outten CE]]
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[[Category: Xue, Y.]]
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[[Category: Xue Y]]
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[[Category: SO4]]
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[[Category: ZN]]
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[[Category: merr family transcriptional regulator]]
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[[Category: zn(ii)-responsive regulator of znta]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:34:27 2008''
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Current revision

Crystal structure of the Zn(II) form of E. coli ZntR, a zinc-sensing transcriptional regulator (space group I4122)

PDB ID 1q09

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