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1q0k

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(New page: 200px<br /><applet load="1q0k" size="450" color="white" frame="true" align="right" spinBox="true" caption="1q0k, resolution 2.10&Aring;" /> '''Crystal structure of...)
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[[Image:1q0k.gif|left|200px]]<br /><applet load="1q0k" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1q0k, resolution 2.10&Aring;" />
 
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'''Crystal structure of Ni-containing superoxide dismutase with Ni-ligation corresponding to the thiosulfate-reduced state'''<br />
 
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==Overview==
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==Crystal structure of Ni-containing superoxide dismutase with Ni-ligation corresponding to the thiosulfate-reduced state==
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Superoxide dismutases (SODs, EC 1.15.1.1) are ubiquitous enzymes that, efficiently catalyze the dismutation of superoxide radical anions to, protect biological molecules from oxidative damage. The crystal structure, of nickel-containing SOD (NiSOD) from Streptomyces seoulensis was, determined for the resting, x-ray-reduced, and thiosulfate-reduced enzyme, state. NiSOD is a homohexamer consisting of four-helix-bundle subunits., The catalytic center resides in the N-terminal active-site loop, where a, Ni(III) ion is coordinated by the amino group of His-1, the amide group of, Cys-2, two thiolate groups of Cys-2 and Cys-6, and the imidazolate of, His-1 as axial ligand that is lost in the chemically reduced state as well, as after x-ray-induced reduction. This structure represents a third class, of SODs concerning the catalytic metal species, subunit structure, and, oligomeric organization. It adds a member to the small number of, Ni-metalloenzymes and contributes with its Ni(III) active site to the, general understanding of Ni-related biochemistry. NiSOD is shown to occur, also in bacteria other than Streptomyces and is predicted to be present in, some cyanobacteria.
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<StructureSection load='1q0k' size='340' side='right'caption='[[1q0k]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1q0k]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_seoulensis Streptomyces seoulensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q0K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Q0K FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=THJ:THIOSULFATE'>THJ</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1q0k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q0k OCA], [https://pdbe.org/1q0k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1q0k RCSB], [https://www.ebi.ac.uk/pdbsum/1q0k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1q0k ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SODN_STRSO SODN_STRSO]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/q0/1q0k_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1q0k ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1Q0K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_seoulensis Streptomyces seoulensis] with NI, SO4 and THJ as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Superoxide_dismutase Superoxide dismutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.15.1.1 1.15.1.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1Q0K OCA].
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*[[Superoxide dismutase 3D structures|Superoxide dismutase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of nickel-containing superoxide dismutase reveals another type of active site., Wuerges J, Lee JW, Yim YI, Yim HS, Kang SO, Carugo KD, Proc Natl Acad Sci U S A. 2004 Jun 8;101(23):8569-74. Epub 2004 Jun 1. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15173586 15173586]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Streptomyces seoulensis]]
[[Category: Streptomyces seoulensis]]
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[[Category: Superoxide dismutase]]
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[[Category: Djinovic Carugo K]]
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[[Category: Carugo, K.Djinovic.]]
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[[Category: Kang S-O]]
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[[Category: Kang, S.O.]]
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[[Category: Lee J-W]]
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[[Category: Lee, J.W.]]
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[[Category: Wuerges J]]
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[[Category: Wuerges, J.]]
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[[Category: Yim H-S]]
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[[Category: Yim, H.S.]]
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[[Category: Yim Y-I]]
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[[Category: Yim, Y.I.]]
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[[Category: NI]]
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[[Category: SO4]]
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[[Category: THJ]]
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[[Category: homohexamer of four-helix bundles]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 00:16:55 2007''
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Current revision

Crystal structure of Ni-containing superoxide dismutase with Ni-ligation corresponding to the thiosulfate-reduced state

PDB ID 1q0k

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