1q1h

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(New page: 200px<br /><applet load="1q1h" size="450" color="white" frame="true" align="right" spinBox="true" caption="1q1h, resolution 2.90&Aring;" /> '''An extended winged h...)
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[[Image:1q1h.jpg|left|200px]]<br /><applet load="1q1h" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1q1h, resolution 2.90&Aring;" />
 
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'''An extended winged helix domain in general transcription factor E/IIE alpha'''<br />
 
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==Overview==
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==An extended winged helix domain in general transcription factor E/IIE alpha==
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Initiation of eukaryotic mRNA transcription requires melting of promoter, DNA with the help of the general transcription factors TFIIE and TFIIH., Here we define a conserved and functionally essential N-terminal domain in, TFE, the archaeal homolog of the large TFIIE subunit alpha. X-ray, crystallography shows that this TFE domain adopts a winged, helix-turn-helix (winged helix) fold, extended by specific alpha-helices, at the N and C termini. Although the winged helix fold is often found in, DNA-binding proteins, we show that TFE is not a typical DNA-binding winged, helix protein, because its putative DNA-binding face shows a negatively, charged groove and an unusually long wing, and because the domain lacks, DNA-binding activity in vitro. The groove and a conserved hydrophobic, surface patch on the additional N-terminal alpha-helix may, however, allow, for interactions with other general transcription factors and RNA, polymerase. Homology modeling shows that the TFE domain is conserved in, TFIIE alpha, including the potential functional surfaces.
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<StructureSection load='1q1h' size='340' side='right'caption='[[1q1h]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1q1h]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus Saccharolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q1H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Q1H FirstGlance]. <br>
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1Q1H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1Q1H OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1q1h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q1h OCA], [https://pdbe.org/1q1h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1q1h RCSB], [https://www.ebi.ac.uk/pdbsum/1q1h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1q1h ProSAT]</span></td></tr>
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==Reference==
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</table>
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An extended winged helix domain in general transcription factor E/IIE alpha., Meinhart A, Blobel J, Cramer P, J Biol Chem. 2003 Nov 28;278(48):48267-74. Epub 2003 Sep 17. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=13679366 13679366]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/TFE_SACS2 TFE_SACS2] Transcription factor that plays a role in the activation of archaeal genes transcribed by RNA polymerase. Facilitates transcription initiation by enhancing TATA-box recognition by TATA-box-binding protein (Tbp), and transcription factor B (Tfb) and RNA polymerase recruitment. Not absolutely required for transcription in vitro, but particularly important in cases where Tbp or Tfb function is not optimal. It dynamically alters the nucleic acid-binding properties of RNA polymerases by stabilizing the initiation complex and destabilizing elongation complexes. Seems to translocate with the RNA polymerase following initiation and acts by binding to the non template strand of the transcription bubble in elongation complexes.<ref>PMID:11258705</ref>
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[[Category: Sulfolobus solfataricus]]
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== Evolutionary Conservation ==
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[[Category: Blobel, J.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Cramer, P.]]
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Check<jmol>
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[[Category: Meinhart, A.]]
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<jmolCheckbox>
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[[Category: preinitiation complex]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/q1/1q1h_consurf.spt"</scriptWhenChecked>
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[[Category: promoter melting]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: rna polymerase ii]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: tfe]]
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</jmolCheckbox>
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[[Category: tfiie]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1q1h ConSurf].
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[[Category: tfiih]]
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<div style="clear:both"></div>
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[[Category: transcription bubble]]
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== References ==
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[[Category: transcription initiation]]
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<references/>
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__TOC__
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 03:24:17 2007''
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Saccharolobus solfataricus]]
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[[Category: Blobel J]]
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[[Category: Cramer P]]
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[[Category: Meinhart A]]

Current revision

An extended winged helix domain in general transcription factor E/IIE alpha

PDB ID 1q1h

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