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1qcu

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CRYSTAL STRUCTURE OF AN 18 BASE PAIR COPY CONTROL RELATED RNA DUPLEX

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Retrieved from "http://52.214.119.220/wiki/index.php/1qcu"

Categories: Large Structures | Klosterman PS | Shah SA | Steitz TA

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-[[Image:1qcu.gif|left|200px]]<br /><applet load="1qcu" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1qcu, resolution 1.20&Aring;" />
-'''CRYSTAL STRUCTURE OF AN 18 BASE PAIR COPY CONTROL RELATED RNA DUPLEX'''<br />
-==Overview==
+==CRYSTAL STRUCTURE OF AN 18 BASE PAIR COPY CONTROL RELATED RNA DUPLEX==
-The structures of two RNA duplexes, whose sequences correspond to portions, of the ColE1 plasmid copy control RNA I and RNA II, have been determined., Crystals containing the 18mers 5'-CA CCGUUGGUAGCGGUGC-3' and, 5'-CACCGCUACCAACGGUGC-3' diffract to 1.20 A resolution while those, containing the 19mers 5'-GCACCGUUGGUAGCGGUGC-3' and, 5'-GCACCGCUACCAACGGUGC-3' diffract to 1.55 A resolution. Both duplexes are, standard A form, with Watson-Crick base pairing throughout. Use of, anisotropic atomic displacement factors in refinement of the 1.20 A, structure dramatically improved refinement statistics, resulting in a, final R(free) of 15.0% and a crystallographic R-factor of 11.6%. Perhaps, surprisingly, these crystals of the 18 base pair RNA exhibit a 36-fold, static disorder, resulting in a structure with a single sugar-phosphate, backbone conformation and an averaged base composition at each residue., Since the sugar-phosphate backbone structure is identical in the 36, different nucleotides that are superimposed, there can be no, sequence-dependent variation in the structure. The average ribose pucker, amplitude is 45.8 degrees for the 18 base pair structure and 46.4 degrees, for the 19 base pair structure; these values are respectively 19% and 20%, larger than the average pucker amplitude reported from nucleoside crystal, structures. A standard RNA water structure, based on analysis of the, hydration of these crystal structures and that of the TAR RNA stem, [Ippolito, J. A., and Steitz, T. A. (1998) Proc. Natl. Acad. Sci. U.S.A., 95, 9819-9824], has been derived, which has allowed us to predict water, positions in lower resolution RNA crystal structures. We report a new RNA, packing motif, in which three pro-S(p) phosphate oxygens interact with an, ammonium ion.
+<StructureSection load='1qcu' size='340' side='right'caption='[[1qcu]], [[Resolution|resolution]] 1.20&Aring;' scene=''>
+== Structural highlights ==
-==About this Structure==
+<table><tr><td colspan='2'>[[1qcu]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QCU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QCU FirstGlance]. <br>
-QCU is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with NH4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QCU OCA].
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.2&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH4:AMMONIUM+ION'>NH4</scene></td></tr>
-==Reference==
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qcu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qcu OCA], [https://pdbe.org/1qcu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qcu RCSB], [https://www.ebi.ac.uk/pdbsum/1qcu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qcu ProSAT]</span></td></tr>
-Crystal structures of two plasmid copy control related RNA duplexes: An 18 base pair duplex at 1.20 A resolution and a 19 base pair duplex at 1.55 A resolution., Klosterman PS, Shah SA, Steitz TA, Biochemistry. 1999 Nov 9;38(45):14784-92. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10555960 10555960]
+</table>
-[[Category: Protein complex]]
+__TOC__
-[[Category: Klosterman, P.S.]]
+</StructureSection>
-[[Category: Shah, S.A.]]
+[[Category: Large Structures]]
-[[Category: Steitz, T.A.]]
+[[Category: Klosterman PS]]
-[[Category: NH4]]
+[[Category: Shah SA]]
-[[Category: a-rna structure]]
+[[Category: Steitz TA]]
-[[Category: ribonucleic acid]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 03:59:02 2007''

1qcu

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CRYSTAL STRUCTURE OF AN 18 BASE PAIR COPY CONTROL RELATED RNA DUPLEX

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