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1qrv

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CRYSTAL STRUCTURE OF THE COMPLEX OF HMG-D AND DNA

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OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1qrv"

Categories: Drosophila melanogaster | Large Structures | Churchill MEA | Murphy IV FV | Sweet RM

@@ Line 1: / Line 1: @@
-[[Image:1qrv.gif|left|200px]]
- {{Structure
+==CRYSTAL STRUCTURE OF THE COMPLEX OF HMG-D AND DNA==
-|PDB= 1qrv |SIZE=350|CAPTION= <scene name='initialview01'>1qrv</scene>, resolution 2.20&Aring;
+<StructureSection load='1qrv' size='340' side='right'caption='[[1qrv]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
-|SITE=
+== Structural highlights ==
-|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>
+<table><tr><td colspan='2'>[[1qrv]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QRV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QRV FirstGlance]. <br>
-|ACTIVITY=
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
-|GENE=
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-|DOMAIN=
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qrv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qrv OCA], [https://pdbe.org/1qrv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qrv RCSB], [https://www.ebi.ac.uk/pdbsum/1qrv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qrv ProSAT]</span></td></tr>
-|RELATEDENTRY=[[2lef|2LEF]], [[1hrz|1HRZ]], [[1hma|1HMA]], [[1hmf|1HMF]], [[1cg7|1CG7]], [[1ckt|1CKT]]
+</table>
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qrv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qrv OCA], [http://www.ebi.ac.uk/pdbsum/1qrv PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1qrv RCSB]</span>
+== Function ==
-}}
+[https://www.uniprot.org/uniprot/HMGD_DROME HMGD_DROME] Binds preferentially single-stranded DNA and unwinds double stranded DNA. Prefers sites containing the sequence 5'-ttg-3'. Facilitates DNA bending. Associated with early embryonic chromatin in the absence of histone H1.<ref>PMID:1373803</ref> <ref>PMID:8168480</ref> <ref>PMID:7720717</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qr/1qrv_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qrv ConSurf].
+<div style="clear:both"></div>
-'''CRYSTAL STRUCTURE OF THE COMPLEX OF HMG-D AND DNA'''
+==See Also==
+*[[High mobility group protein|High mobility group protein]]
+== References ==
-==Overview==
+<references/>
-The high mobility group (HMG) chromosomal proteins, which are common to all eukaryotes, bind DNA in a non-sequence-specific fashion to promote chromatin function and gene regulation. They interact directly with nucleosomes and are believed to be modulators of chromatin structure. They are also important in V(D)J recombination and in activating a number of regulators of gene expression, including p53, Hox transcription factors and steroid hormone receptors, by increasing their affinity for DNA. The X-ray crystal structure, at 2.2 A resolution, of the HMG domain of the Drosophila melanogaster protein, HMG-D, bound to DNA provides the first detailed view of a chromosomal HMG domain interacting with linear DNA and reveals the molecular basis of non-sequence-specific DNA recognition. Ser10 forms water-mediated hydrogen bonds to DNA bases, and Val32 with Thr33 partially intercalates the DNA. These two 'sequence-neutral' mechanisms of DNA binding substitute for base-specific hydrogen bonds made by equivalent residues of the sequence-specific HMG domain protein, lymphoid enhancer factor-1. The use of multiple intercalations and water-mediated DNA contacts may prove to be generally important mechanisms by which chromosomal proteins bind to DNA in the minor groove.
+__TOC__
+</StructureSection>
-==About this Structure==
-QRV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QRV OCA].
-==Reference==
-The structure of a chromosomal high mobility group protein-DNA complex reveals sequence-neutral mechanisms important for non-sequence-specific DNA recognition., Murphy FV 4th, Sweet RM, Churchill ME, EMBO J. 1999 Dec 1;18(23):6610-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10581235 10581235]
 [[Category: Drosophila melanogaster]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Churchill, M E.A.]]
+[[Category: Churchill MEA]]
-[[Category: IV, F V.Murphy.]]
+[[Category: Murphy IV FV]]
-[[Category: Sweet, R M.]]
+[[Category: Sweet RM]]
-[[Category: hmg domain]]
-[[Category: non-sequence specific chromosomal protein hmg-d]]
-[[Category: protein-dna complex]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:18:07 2008''

1qrv

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CRYSTAL STRUCTURE OF THE COMPLEX OF HMG-D AND DNA

Structural highlights

Function

Evolutionary Conservation

See Also

References

Contents

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