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1qwy

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Latent LytM at 1.3 A resolution

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Retrieved from "http://52.214.119.220/wiki/index.php/1qwy"

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-[[Image:1qwy.jpg|left|200px]]<br /><applet load="1qwy" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1qwy, resolution 1.30&Aring;" />
-'''Latent LytM at 1.3 A resolution'''<br />
-==Overview==
+==Latent LytM at 1.3 A resolution==
-LytM, an autolysin from Staphylococcus aureus, is a Zn(2+)-dependent, glycyl-glycine endopeptidase with a characteristic HxH motif that belongs, to the lysostaphin-type (MEROPS M23/37) of metallopeptidases. Here, we, present the 1.3A crystal structure of LytM, the first structure of a, lysostaphin-type peptidase. In the LytM structure, the Zn(2+) is, tetrahedrally coordinated by the side-chains of N117, H210, D214 and H293, the second histidine of the HxH motif. Although close to the active-site, H291, the first histidine of the HxH motif, is not directly involved in, Zn(2+)-coordination, and there is no water molecule in the coordination, sphere of the Zn(2+), suggesting that the crystal structure shows a latent, form of the enzyme. Although LytM has not previously been considered as a, proenzyme, we show that a truncated version of LytM that lacks the, N-terminal part with the poorly conserved Zn(2+) ligand N117 has much, higher specific activity than full-length enzyme. This observation is, consistent with the known removal of profragments in other, lysostaphin-type proteins and with a prior observation of an active LytM, degradation fragment in S.aureus supernatant. The "asparagine switch" in, LytM is analogous to the "cysteine switch" in pro-matrix metalloproteases.
+<StructureSection load='1qwy' size='340' side='right'caption='[[1qwy]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
+== Structural highlights ==
-==About this Structure==
+<table><tr><td colspan='2'>[[1qwy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_NCTC_8325 Staphylococcus aureus subsp. aureus NCTC 8325]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QWY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QWY FirstGlance]. <br>
-QWY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QWY OCA].
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-==Reference==
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qwy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qwy OCA], [https://pdbe.org/1qwy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qwy RCSB], [https://www.ebi.ac.uk/pdbsum/1qwy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qwy ProSAT]</span></td></tr>
-Latent LytM at 1.3A resolution., Odintsov SG, Sabala I, Marcyjaniak M, Bochtler M, J Mol Biol. 2004 Jan 16;335(3):775-85. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14687573 14687573]
+</table>
-[[Category: Single protein]]
+== Function ==
-[[Category: Staphylococcus aureus]]
+[https://www.uniprot.org/uniprot/LYTM_STAA8 LYTM_STAA8] Peptidoglycan hydrolase (autolysin) specifically acting on polyglycine interpeptide bridges of the cell wall peptidoglycan.<ref>PMID:10220159</ref> <ref>PMID:8095258</ref>
-[[Category: Bochtler, M.]]
+== Evolutionary Conservation ==
-[[Category: Marcyjaniak, M.]]
+[[Image:Consurf_key_small.gif|200px|right]]
-[[Category: Odintsov, S.G.]]
+Check<jmol>
-[[Category: Sabala, I.]]
+  <jmolCheckbox>
-[[Category: ZN]]
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qw/1qwy_consurf.spt"</scriptWhenChecked>
-[[Category: lytm lysostaphin metalloprotease asparagine switch]]
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 01:04:52 2007''
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qwy ConSurf].
+<div style="clear:both"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Staphylococcus aureus subsp. aureus NCTC 8325]]
+[[Category: Bochtler M]]
+[[Category: Marcyjaniak M]]
+[[Category: Odintsov SG]]
+[[Category: Sabala I]]

1qwy

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Latent LytM at 1.3 A resolution

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