This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1qzz

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:19, 14 February 2024) (edit) (undo)
 
(7 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1qzz.png|left|200px]]
 
-
{{STRUCTURE_1qzz| PDB=1qzz | SCENE= }}
+
==Crystal structure of aclacinomycin-10-hydroxylase (RdmB) in complex with S-adenosyl-L-methionine (SAM)==
-
 
+
<StructureSection load='1qzz' size='340' side='right'caption='[[1qzz]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
-
===Crystal structure of aclacinomycin-10-hydroxylase (RdmB) in complex with S-adensyl-L-methionine (SAM)===
+
== Structural highlights ==
-
 
+
<table><tr><td colspan='2'>[[1qzz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_purpurascens Streptomyces purpurascens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QZZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QZZ FirstGlance]. <br>
-
{{ABSTRACT_PUBMED_14607118}}
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
-
 
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
-
==About this Structure==
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qzz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qzz OCA], [https://pdbe.org/1qzz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qzz RCSB], [https://www.ebi.ac.uk/pdbsum/1qzz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qzz ProSAT]</span></td></tr>
-
[[1qzz]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_purpurascens Streptomyces purpurascens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QZZ OCA].
+
</table>
-
 
+
== Function ==
-
==Reference==
+
[https://www.uniprot.org/uniprot/RDMB_STREF RDMB_STREF] Involved in the biosynthesis of the anthracycline aclacinomycin which is an aromatic polyketide antibiotic that exhibits high cytotoxicity and is widely applied in the chemotherapy of a variety of cancers. In vivo and in vitro, RdmB catalyzes the removal of the carboxylic group from the C-10 position of 15-demethoxyaclacinomycin T coupled to hydroxylation at the same C-10 position. It could also catalyze the removal of the carboxylic group at the C-10 position of 15-demethoxy-epsilon-rhodomycin coupled to hydroxylation at the same C-10 position to yield rhodomycin B. The reaction catalyzes by RdmB is intriguing, since the enzyme does not use any of the cofactors usually associated with hydroxylases such as flavins and/or metal ions to activate molecular oxygen.<ref>PMID:11004563</ref> <ref>PMID:15548527</ref>
-
<ref group="xtra">PMID:014607118</ref><references group="xtra"/>
+
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qz/1qzz_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qzz ConSurf].
 +
<div style="clear:both"></div>
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
[[Category: Streptomyces purpurascens]]
[[Category: Streptomyces purpurascens]]
-
[[Category: Jansson, A.]]
+
[[Category: Jansson A]]
-
[[Category: Lindqvist, Y.]]
+
[[Category: Lindqvist Y]]
-
[[Category: Mantsala, P.]]
+
[[Category: Mantsala P]]
-
[[Category: Niemi, J.]]
+
[[Category: Niemi J]]
-
[[Category: SPINE, Structural Proteomics in Europe.]]
+
[[Category: Schneider G]]
-
[[Category: Schneider, G.]]
+
-
[[Category: Anthracycline]]
+
-
[[Category: Hydroxylase]]
+
-
[[Category: Methyltransferase]]
+
-
[[Category: Oxidoreductase]]
+
-
[[Category: Polyketide]]
+
-
[[Category: Spine]]
+
-
[[Category: Streptomyce]]
+
-
[[Category: Structural genomic]]
+
-
[[Category: Structural proteomics in europe]]
+
-
[[Category: Tailoring enzyme]]
+
-
[[Category: Transferase]]
+

Current revision

Crystal structure of aclacinomycin-10-hydroxylase (RdmB) in complex with S-adenosyl-L-methionine (SAM)

PDB ID 1qzz

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1qzz"
Personal tools