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1r5x

From Proteopedia

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[[Image:1r5x.gif|left|200px]]
 
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==JAMM: A Metalloprotease-like Zinc Site in the Proteasome and Signalosome==
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The line below this paragraph, containing "STRUCTURE_1r5x", creates the "Structure Box" on the page.
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<StructureSection load='1r5x' size='340' side='right'caption='[[1r5x]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1r5x]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus_DSM_4304 Archaeoglobus fulgidus DSM 4304]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R5X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1R5X FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_1r5x| PDB=1r5x | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1r5x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r5x OCA], [https://pdbe.org/1r5x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1r5x RCSB], [https://www.ebi.ac.uk/pdbsum/1r5x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1r5x ProSAT]</span></td></tr>
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</table>
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'''JAMM: A Metalloprotease-like Zinc Site in the Proteasome and Signalosome'''
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== Function ==
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[https://www.uniprot.org/uniprot/O28085_ARCFU O28085_ARCFU]
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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The JAMM (JAB1/MPN/Mov34 metalloenzyme) motif in Rpn11 and Csn5 underlies isopeptidase activities intrinsic to the proteasome and signalosome, respectively. We show here that the archaebacterial protein AfJAMM possesses the key features of a zinc metalloprotease, yet with a distinct fold. The histidine and aspartic acid of the conserved EX(n)HS/THX(7)SXXD motif coordinate a zinc, whereas the glutamic acid hydrogen-bonds an aqua ligand. By analogy to the active site of thermolysin, we predict that the glutamic acid serves as an acid-base catalyst and the second serine stabilizes a tetrahedral intermediate. Mutagenesis of Csn5 confirms these residues are required for Nedd8 isopeptidase activity. The active site-like architecture specified by the JAMM motif motivates structure-based approaches to the study of JAMM domain proteins and the development of therapeutic proteasome and signalosome inhibitors.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r5/1r5x_consurf.spt"</scriptWhenChecked>
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1R5X is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus_dsm_4304 Archaeoglobus fulgidus dsm 4304]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R5X OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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JAMM: a metalloprotease-like zinc site in the proteasome and signalosome., Ambroggio XI, Rees DC, Deshaies RJ, PLoS Biol. 2004 Jan;2(1):E2. Epub 2003 Nov 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14737182 14737182]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1r5x ConSurf].
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[[Category: Archaeoglobus fulgidus dsm 4304]]
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<div style="clear:both"></div>
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[[Category: Single protein]]
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__TOC__
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[[Category: Ambroggio, X I.]]
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</StructureSection>
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[[Category: Deshaies, R J.]]
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[[Category: Archaeoglobus fulgidus DSM 4304]]
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[[Category: Rees, D C.]]
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[[Category: Large Structures]]
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[[Category: Csn5]]
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[[Category: Ambroggio XI]]
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[[Category: Jab1]]
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[[Category: Deshaies RJ]]
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[[Category: Jamm]]
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[[Category: Rees DC]]
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[[Category: Mov34]]
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[[Category: Mpn]]
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[[Category: Proteasome]]
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[[Category: Rpn11]]
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[[Category: Signalosome]]
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[[Category: Structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:07:18 2008''
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Current revision

JAMM: A Metalloprotease-like Zinc Site in the Proteasome and Signalosome

PDB ID 1r5x

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