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1r94

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(New page: 200px<br /><applet load="1r94" size="450" color="white" frame="true" align="right" spinBox="true" caption="1r94, resolution 2.30&Aring;" /> '''Crystal Structure of...)
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[[Image:1r94.jpg|left|200px]]<br /><applet load="1r94" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1r94, resolution 2.30&Aring;" />
 
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'''Crystal Structure of IscA (MERCURY DERIVATIVE)'''<br />
 
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==Overview==
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==Crystal Structure of IscA (MERCURY DERIVATIVE)==
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IscA belongs to an ancient family of proteins responsible for iron-sulfur, cluster assembly in essential metabolic pathways preserved throughout, evolution. We report here the 2.3 A resolution crystal structure of, Escherichia coli IscA, a novel fold in which mixed beta-sheets form a, compact alpha-beta sandwich domain. In contrast to the highly mobile, secondary structural elements within the bacterial Fe-S scaffold protein, IscU, a protein which is thought to have a similar function, the great, majority of the amino acids that are conserved in IscA homologues are, located in elements that constitute a well-ordered fold. However, the, 10-residue C-terminal tail segment that contains two invariant cysteines, critical for the Fe-S-binding function of a cyanobacterial (Synechocystis, PCC) IscA homologue is not ordered in our structure. In addition, the, crystal packing reveals a helical assembly that is constructed from two, possible tetrameric oligomers of IscA.
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<StructureSection load='1r94' size='340' side='right'caption='[[1r94]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1r94]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R94 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1R94 FirstGlance]. <br>
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1R94 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with HG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1R94 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1r94 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r94 OCA], [https://pdbe.org/1r94 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1r94 RCSB], [https://www.ebi.ac.uk/pdbsum/1r94 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1r94 ProSAT]</span></td></tr>
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Crystal structure of the ancient, Fe-S scaffold IscA reveals a novel protein fold., Bilder PW, Ding H, Newcomer ME, Biochemistry. 2004 Jan 13;43(1):133-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14705938 14705938]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ISCA_ECOLI ISCA_ECOLI] Is able to transfer iron-sulfur clusters to apo-ferredoxin. Multiple cycles of [2Fe2S] cluster formation and transfer are observed, suggesting that IscA acts catalytically. Recruits intracellular free iron so as to provide iron for the assembly of transient iron-sulfur cluster in IscU in the presence of IscS, L-cysteine and the thioredoxin reductase system TrxA/TrxB.[HAMAP-Rule:MF_01429]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r9/1r94_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1r94 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Bilder, P.W.]]
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[[Category: Bilder PW]]
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[[Category: Ding, H.]]
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[[Category: Ding H]]
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[[Category: Newcomer, M.E.]]
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[[Category: Newcomer ME]]
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[[Category: HG]]
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[[Category: beta barrel]]
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[[Category: iron-sulfur cluster protein]]
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[[Category: pseudo-symmetric motifs]]
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[[Category: tetrameric]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 01:21:44 2007''
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Current revision

Crystal Structure of IscA (MERCURY DERIVATIVE)

PDB ID 1r94

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