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1rh1

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[[Image:1rh1.gif|left|200px]]
 
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{{Structure
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==crystal structure of the cytotoxic bacterial protein colicin B at 2.5 A resolution==
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|PDB= 1rh1 |SIZE=350|CAPTION= <scene name='initialview01'>1rh1</scene>, resolution 2.5&Aring;
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<StructureSection load='1rh1' size='340' side='right'caption='[[1rh1]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1rh1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RH1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RH1 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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|GENE= CBA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rh1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rh1 OCA], [https://pdbe.org/1rh1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rh1 RCSB], [https://www.ebi.ac.uk/pdbsum/1rh1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rh1 ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rh1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rh1 OCA], [http://www.ebi.ac.uk/pdbsum/1rh1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1rh1 RCSB]</span>
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[https://www.uniprot.org/uniprot/CEAB_ECOLX CEAB_ECOLX]
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}}
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'''crystal structure of the cytotoxic bacterial protein colicin B at 2.5 A resolution'''
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==See Also==
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*[[Colicin 3D structures|Colicin 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Colicin B (55 kDa) is a cytotoxic protein that recognizes the outer membrane transporter, FepA, as a receptor and, after gaining access to the cytoplasmic membranes of sensitive Escherichia coli cells, forms a pore that depletes the electrochemical potential of the membrane and ultimately results in cell death. To begin to understand the series of dynamic conformational changes that must occur as colicin B translocates from outer membrane to cytoplasmic membrane, we report here the crystal structure of colicin B at 2.5 A resolution. The crystal belongs to the space group C2221 with unit cell dimensions a = 132.162 A, b = 138.167 A, c = 106.16 A. The overall structure of colicin B is dumbbell shaped. Unlike colicin Ia, the only other TonB-dependent colicin crystallized to date, colicin B does not have clearly structurally delineated receptor-binding and translocation domains. Instead, the unique N-terminal lobe of the dumbbell contains both domains and consists of a large (290 residues), mostly beta-stranded structure with two short alpha-helices. This is followed by a single long ( approximately 74 A) helix that connects the N-terminal domain to the C-terminal pore-forming domain, which is composed of 10 alpha-helices arranged in a bundle-type structure, similar to the pore-forming domains of other colicins. The TonB box sequence at the N-terminus folds back to interact with the N-terminal lobe of the dumbbell and leaves the flanking sequences highly disordered. Comparison of sequences among many colicins has allowed the identification of a putative receptor-binding domain.
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==About this Structure==
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1RH1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RH1 OCA].
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==Reference==
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Crystal structure of the cytotoxic bacterial protein colicin B at 2.5 A resolution., Hilsenbeck JL, Park H, Chen G, Youn B, Postle K, Kang C, Mol Microbiol. 2004 Feb;51(3):711-20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14731273 14731273]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Chen, G.]]
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[[Category: Chen G]]
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[[Category: Hilsenbeck, J L.]]
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[[Category: Hilsenbeck JL]]
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[[Category: Kang, C.]]
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[[Category: Kang C]]
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[[Category: Park, H.]]
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[[Category: Park H]]
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[[Category: Postle, K.]]
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[[Category: Postle K]]
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[[Category: Youn, B.]]
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[[Category: Youn B]]
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[[Category: colicin b]]
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[[Category: crystal structure]]
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[[Category: cytotoxic bacterial protein]]
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[[Category: fepa]]
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[[Category: tonb]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:28:11 2008''
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Current revision

crystal structure of the cytotoxic bacterial protein colicin B at 2.5 A resolution

PDB ID 1rh1

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