This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1rh6

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:23, 14 February 2024) (edit) (undo)
 
(One intermediate revision not shown.)
Line 3: Line 3:
<StructureSection load='1rh6' size='340' side='right'caption='[[1rh6]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
<StructureSection load='1rh6' size='340' side='right'caption='[[1rh6]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[1rh6]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacteriophage_lambda Bacteriophage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RH6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RH6 FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[1rh6]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_Lambda Escherichia virus Lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RH6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RH6 FirstGlance]. <br>
-
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">xis ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10710 Bacteriophage lambda])</td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rh6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rh6 OCA], [https://pdbe.org/1rh6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rh6 RCSB], [https://www.ebi.ac.uk/pdbsum/1rh6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rh6 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rh6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rh6 OCA], [https://pdbe.org/1rh6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rh6 RCSB], [https://www.ebi.ac.uk/pdbsum/1rh6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rh6 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
-
[[https://www.uniprot.org/uniprot/VXIS_LAMBD VXIS_LAMBD]] Excisionase and integrase are necessary for the excision of prophage from the host genome by site-specific recombination at the att site.
+
[https://www.uniprot.org/uniprot/VXIS_LAMBD VXIS_LAMBD] Excisionase and integrase are necessary for the excision of prophage from the host genome by site-specific recombination at the att site.
-
<div style="background-color:#fffaf0;">
+
-
== Publication Abstract from PubMed ==
+
-
The excisionase (Xis) protein from bacteriophage lambda is the best characterized member of a large family of recombination directionality factors that control integrase-mediated DNA rearrangements. It triggers phage excision by cooperatively binding to sites X1 and X2 within the phage, bending DNA significantly and recruiting the phage-encoded integrase (Int) protein to site P2. We have determined the co-crystal structure of Xis with its X2 DNA-binding site at 1.7A resolution. Xis forms a unique winged-helix motif that interacts with the major and minor grooves of its binding site using an alpha-helix and an ordered beta-hairpin (wing), respectively. Recognition is achieved through an elaborate water-mediated hydrogen-bonding network at the major groove interface, while the preformed hairpin forms largely non-specific interactions with the minor groove. The structure of the complex provides insights into how Xis recruits Int cooperatively, and suggests a plausible mechanism by which it may distort longer DNA fragments significantly. It reveals a surface on the protein that is likely to mediate Xis-Xis interactions required for its cooperative binding to DNA.
+
-
 
+
-
Crystal structure of the excisionase-DNA complex from bacteriophage lambda.,Sam MD, Cascio D, Johnson RC, Clubb RT J Mol Biol. 2004 Apr 23;338(2):229-40. PMID:15066428<ref>PMID:15066428</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
<div class="pdbe-citations 1rh6" style="background-color:#fffaf0;"></div>
+
-
== References ==
+
-
<references/>
+
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Bacteriophage lambda]]
+
[[Category: Escherichia virus Lambda]]
[[Category: Large Structures]]
[[Category: Large Structures]]
-
[[Category: Cascio, D]]
+
[[Category: Cascio D]]
-
[[Category: Clubb, R T]]
+
[[Category: Clubb RT]]
-
[[Category: Johnson, R C]]
+
[[Category: Johnson RC]]
-
[[Category: Sam, M D]]
+
[[Category: Sam MD]]
-
[[Category: Winged -helix protein]]
+
-
[[Category: Dna architectural protein]]
+
-
[[Category: Dna binding protein-dna complex]]
+
-
[[Category: Phage excision]]
+
-
[[Category: Protein-dna complex]]
+
-
[[Category: Site-specific dna recombination]]
+

Current revision

Bacteriophage Lambda Excisionase (Xis)-DNA Complex

PDB ID 1rh6

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1rh6"
Personal tools