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1rh6

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(New page: 200px<br /><applet load="1rh6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rh6, resolution 1.70&Aring;" /> '''Bacteriophage Lambda...)
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[[Image:1rh6.gif|left|200px]]<br /><applet load="1rh6" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1rh6, resolution 1.70&Aring;" />
 
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'''Bacteriophage Lambda Excisionase (Xis)-DNA Complex'''<br />
 
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==Overview==
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==Bacteriophage Lambda Excisionase (Xis)-DNA Complex==
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The excisionase (Xis) protein from bacteriophage lambda is the best, characterized member of a large family of recombination directionality, factors that control integrase-mediated DNA rearrangements. It triggers, phage excision by cooperatively binding to sites X1 and X2 within the, phage, bending DNA significantly and recruiting the phage-encoded, integrase (Int) protein to site P2. We have determined the co-crystal, structure of Xis with its X2 DNA-binding site at 1.7A resolution. Xis, forms a unique winged-helix motif that interacts with the major and minor, grooves of its binding site using an alpha-helix and an ordered, beta-hairpin (wing), respectively. Recognition is achieved through an, elaborate water-mediated hydrogen-bonding network at the major groove, interface, while the preformed hairpin forms largely non-specific, interactions with the minor groove. The structure of the complex provides, insights into how Xis recruits Int cooperatively, and suggests a plausible, mechanism by which it may distort longer DNA fragments significantly. It, reveals a surface on the protein that is likely to mediate Xis-Xis, interactions required for its cooperative binding to DNA.
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<StructureSection load='1rh6' size='340' side='right'caption='[[1rh6]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1rh6]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_Lambda Escherichia virus Lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RH6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RH6 FirstGlance]. <br>
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1RH6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1RH6 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rh6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rh6 OCA], [https://pdbe.org/1rh6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rh6 RCSB], [https://www.ebi.ac.uk/pdbsum/1rh6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rh6 ProSAT]</span></td></tr>
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==Reference==
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</table>
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Crystal structure of the excisionase-DNA complex from bacteriophage lambda., Sam MD, Cascio D, Johnson RC, Clubb RT, J Mol Biol. 2004 Apr 23;338(2):229-40. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15066428 15066428]
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== Function ==
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[[Category: Enterobacteria phage lambda]]
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[https://www.uniprot.org/uniprot/VXIS_LAMBD VXIS_LAMBD] Excisionase and integrase are necessary for the excision of prophage from the host genome by site-specific recombination at the att site.
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[[Category: Single protein]]
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__TOC__
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[[Category: Cascio, D.]]
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</StructureSection>
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[[Category: Clubb, R.T.]]
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[[Category: Escherichia virus Lambda]]
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[[Category: Johnson, R.C.]]
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[[Category: Large Structures]]
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[[Category: Sam, M.D.]]
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[[Category: Cascio D]]
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[[Category: 'winged'-helix protein]]
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[[Category: Clubb RT]]
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[[Category: dna architectural protein]]
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[[Category: Johnson RC]]
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[[Category: phage excision]]
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[[Category: Sam MD]]
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[[Category: protein-dna complex]]
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[[Category: site-specific dna recombination]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 01:35:41 2007''
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Current revision

Bacteriophage Lambda Excisionase (Xis)-DNA Complex

PDB ID 1rh6

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