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1rk8

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(New page: 200px<br /><applet load="1rk8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rk8, resolution 1.90&Aring;" /> '''Structure of the cyt...)
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[[Image:1rk8.gif|left|200px]]<br /><applet load="1rk8" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1rk8, resolution 1.90&Aring;" />
 
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'''Structure of the cytosolic protein PYM bound to the Mago-Y14 core of the exon junction complex'''<br />
 
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==Overview==
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==Structure of the cytosolic protein PYM bound to the Mago-Y14 core of the exon junction complex==
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The exon junction complex (EJC) is deposited on mRNAs as a consequence of, splicing and influences postsplicing mRNA metabolism. The Mago-Y14, heterodimer is a core component of the EJC. Recently, the protein PYM has, been identified as an interacting partner of Mago-Y14. Here we show that, PYM is a cytoplasmic RNA-binding protein that is excluded from the nucleus, by Crm1. PYM interacts directly with Mago-Y14 by means of its N-terminal, domain. The crystal structure of the Drosophila ternary complex at 1.9 A, resolution reveals that PYM binds Mago and Y14 simultaneously, capping, their heterodimerization interface at conserved surface residues., Formation of this ternary complex is also observed with the human, proteins. Mago residues involved in the interaction with PYM have been, implicated in nonsense-mediated mRNA decay (NMD). Consistently, human PYM, is active in NMD tethering assays. Together, these data suggest a role for, PYM in NMD.
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<StructureSection load='1rk8' size='340' side='right'caption='[[1rk8]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1rk8]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RK8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RK8 FirstGlance]. <br>
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1RK8 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1RK8 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rk8 OCA], [https://pdbe.org/1rk8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rk8 RCSB], [https://www.ebi.ac.uk/pdbsum/1rk8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rk8 ProSAT]</span></td></tr>
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Molecular insights into the interaction of PYM with the Mago-Y14 core of the exon junction complex., Bono F, Ebert J, Unterholzner L, Guttler T, Izaurralde E, Conti E, EMBO Rep. 2004 Mar;5(3):304-10. Epub 2004 Feb 13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14968132 14968132]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MGN_DROME MGN_DROME] Participates in the bidirectional intercellular signaling between the posterior follicle cells and oocyte to establish spatial coordinates that induces axis formation. Complex with tsu is essential for cytoplasmic localization of oskar in the posterior pole of oocytes. Required for the polarization of the oocyte microtubule cytoskeleton.<ref>PMID:8026338</ref> <ref>PMID:9272960</ref> <ref>PMID:9210377</ref> <ref>PMID:12730685</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rk/1rk8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rk8 ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Drosophila melanogaster]]
[[Category: Drosophila melanogaster]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Bono, F.]]
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[[Category: Bono F]]
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[[Category: Conti, E.]]
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[[Category: Conti E]]
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[[Category: Ebert, J.]]
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[[Category: Ebert J]]
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[[Category: Guettler, T.]]
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[[Category: Guettler T]]
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[[Category: Izaurralde, E.]]
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[[Category: Izaurralde E]]
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[[Category: CA]]
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[[Category: mrna processing; rrm; rbd; nmd; oskar mrna localization]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 01:39:44 2007''
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Current revision

Structure of the cytosolic protein PYM bound to the Mago-Y14 core of the exon junction complex

PDB ID 1rk8

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