1rkc

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[[Image:1rkc.gif|left|200px]]
 
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{{Structure
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==Human vinculin head (1-258) in complex with talin's vinculin binding site 3 (residues 1944-1969)==
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|PDB= 1rkc |SIZE=350|CAPTION= <scene name='initialview01'>1rkc</scene>, resolution 2.70&Aring;
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<StructureSection load='1rkc' size='340' side='right'caption='[[1rkc]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1rkc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RKC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RKC FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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|GENE= VCL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rkc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rkc OCA], [https://pdbe.org/1rkc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rkc RCSB], [https://www.ebi.ac.uk/pdbsum/1rkc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rkc ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=[[1rke|1RKE]]
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== Disease ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rkc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rkc OCA], [http://www.ebi.ac.uk/pdbsum/1rkc PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1rkc RCSB]</span>
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[https://www.uniprot.org/uniprot/VINC_HUMAN VINC_HUMAN] Defects in VCL are the cause of cardiomyopathy dilated type 1W (CMD1W) [MIM:[https://omim.org/entry/611407 611407]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.<ref>PMID:11815424</ref> <ref>PMID:16236538</ref> Defects in VCL are the cause of familial hypertrophic cardiomyopathy type 15 (CMH15) [MIM:[https://omim.org/entry/613255 613255]. It is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.<ref>PMID:16712796</ref>
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}}
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== Function ==
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[https://www.uniprot.org/uniprot/VINC_HUMAN VINC_HUMAN] Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.<ref>PMID:20484056</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rk/1rkc_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rkc ConSurf].
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<div style="clear:both"></div>
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'''Human vinculin head (1-258) in complex with talin's vinculin binding site 3 (residues 1944-1969)'''
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==See Also==
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*[[Talin 3D structures|Talin 3D structures]]
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*[[Vinculin|Vinculin]]
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==Overview==
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== References ==
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Vinculin is a conserved component and an essential regulator of both cell-cell (cadherin-mediated) and cell-matrix (integrin-talin-mediated focal adhesions) junctions, and it anchors these adhesion complexes to the actin cytoskeleton by binding to talin in integrin complexes or to alpha-actinin in cadherin junctions. In its resting state, vinculin is held in a closed conformation through interactions between its head (Vh) and tail (Vt) domains. The binding of vinculin to focal adhesions requires its association with talin. Here we report the crystal structures of human vinculin in its inactive and talin-activated states. Talin binding induces marked conformational changes in Vh, creating a novel helical bundle structure, and this alteration actively displaces Vt from Vh. These results, as well as the ability of alpha-actinin to also bind to Vh and displace Vt from pre-existing Vh-Vt complexes, support a model whereby Vh functions as a domain that undergoes marked structural changes that allow vinculin to direct cytoskeletal assembly in focal adhesions and adherens junctions. Notably, talin's effects on Vh structure establish helical bundle conversion as a signalling mechanism by which proteins direct cellular responses.
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<references/>
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__TOC__
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==Disease==
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</StructureSection>
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Known disease associated with this structure: Cardiomyopathy, dilated, 1W OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=193065 193065]]
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==About this Structure==
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1RKC is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RKC OCA].
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==Reference==
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Vinculin activation by talin through helical bundle conversion., Izard T, Evans G, Borgon RA, Rush CL, Bricogne G, Bois PR, Nature. 2004 Jan 8;427(6970):171-5. Epub 2003 Dec 31. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14702644 14702644]
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[[Category: Gallus gallus]]
[[Category: Gallus gallus]]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Bois, P R.]]
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[[Category: Bois PR]]
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[[Category: Borgon, R A.]]
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[[Category: Borgon RA]]
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[[Category: Bricogne, G.]]
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[[Category: Bricogne G]]
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[[Category: Evans, G.]]
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[[Category: Evans G]]
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[[Category: Izard, T.]]
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[[Category: Izard T]]
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[[Category: Rush, C L.]]
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[[Category: Rush CL]]
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[[Category: actin-binding]]
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[[Category: cytoskeleton]]
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[[Category: x-ray crystallography]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:29:22 2008''
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Current revision

Human vinculin head (1-258) in complex with talin's vinculin binding site 3 (residues 1944-1969)

PDB ID 1rkc

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