1s9h

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[[Image:1s9h.gif|left|200px]]
 
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==Crystal Structure of Adeno-associated virus Type 2 Rep40==
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The line below this paragraph, containing "STRUCTURE_1s9h", creates the "Structure Box" on the page.
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<StructureSection load='1s9h' size='340' side='right'caption='[[1s9h]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1s9h]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Adeno-associated_virus_2 Adeno-associated virus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S9H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S9H FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s9h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s9h OCA], [https://pdbe.org/1s9h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s9h RCSB], [https://www.ebi.ac.uk/pdbsum/1s9h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s9h ProSAT]</span></td></tr>
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{{STRUCTURE_1s9h| PDB=1s9h | SCENE= }}
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</table>
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== Function ==
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'''Crystal Structure of Adeno-associated virus Type 2 Rep40'''
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[https://www.uniprot.org/uniprot/REP68_AAV2S REP68_AAV2S] Plays an essential role in the initiation of viral DNA synthesis. Binds specifically to an inverted terminal repeat element (ITR) on the 3' and 5' ends of the viral DNA, where it cleaves a site specifically to generate a priming site for initiation of the synthesis of a complementary strand. Plays also a role as transcriptional regulator, DNA helicase and as key factor in site-specific integration of the viral genome. Inhibits the host cell cycle G1/S and G2/M transitions. These arrests may provide essential cellular factors for viral DNA replication.<ref>PMID:9882364</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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We report here the crystal structure of an SF3 DNA helicase, Rep40, from adeno-associated virus 2 (AAV2). We show that AAV2 Rep40 is structurally more similar to the AAA(+) class of cellular proteins than to DNA helicases from other superfamilies. The structure delineates the expected Walker A and B motifs, but also reveals an unexpected "arginine finger" that directly implies the requirement of Rep40 oligomerization for ATP hydrolysis and helicase activity. Further, the Rep40 AAA(+) domain is novel in that it is unimodular as opposed to bimodular. Altogether, the structural connection to AAA(+) proteins defines the general architecture of SF3 DNA helicases, a family that includes simian virus 40 (SV40) T antigen, as well as provides a conceptual framework for understanding the role of Rep proteins during AAV DNA replication, packaging, and site-specific integration.
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/s9/1s9h_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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1S9H is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Adeno-associated_virus_2 Adeno-associated virus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S9H OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1s9h ConSurf].
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Crystal structure of the SF3 helicase from adeno-associated virus type 2., James JA, Escalante CR, Yoon-Robarts M, Edwards TA, Linden RM, Aggarwal AK, Structure. 2003 Aug;11(8):1025-35. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12906833 12906833]
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Adeno-associated virus 2]]
[[Category: Adeno-associated virus 2]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Aggarwal, A K.]]
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[[Category: Aggarwal AK]]
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[[Category: Edwards, T A.]]
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[[Category: Edwards TA]]
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[[Category: Escalante, C R.]]
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[[Category: Escalante CR]]
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[[Category: James, J A.]]
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[[Category: James JA]]
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[[Category: Linden, R M.]]
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[[Category: Linden RM]]
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[[Category: Yoon-Robarts, M.]]
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[[Category: Yoon-Robarts M]]
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[[Category: Aaa+ protein]]
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[[Category: Helicase]]
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[[Category: P-loop]]
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[[Category: Sensor 1]]
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[[Category: Walker some]]
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[[Category: Walker b]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:27:00 2008''
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Current revision

Crystal Structure of Adeno-associated virus Type 2 Rep40

PDB ID 1s9h

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