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| - | [[Image:1sa3.gif|left|200px]] | |
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| - | {{Structure
| + | ==An asymmetric complex of restriction endonuclease MspI on its palindromic DNA recognition site== |
| - | |PDB= 1sa3 |SIZE=350|CAPTION= <scene name='initialview01'>1sa3</scene>, resolution 1.95Å
| + | <StructureSection load='1sa3' size='340' side='right'caption='[[1sa3]], [[Resolution|resolution]] 1.95Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>
| + | <table><tr><td colspan='2'>[[1sa3]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Moraxella_sp. Moraxella sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SA3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SA3 FirstGlance]. <br> |
| - | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span>
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95Å</td></tr> |
| - | |GENE= MSPIR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=479 Moraxella sp.])
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> |
| - | |DOMAIN=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sa3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sa3 OCA], [https://pdbe.org/1sa3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sa3 RCSB], [https://www.ebi.ac.uk/pdbsum/1sa3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sa3 ProSAT]</span></td></tr> |
| - | |RELATEDENTRY= | + | </table> |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sa3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sa3 OCA], [http://www.ebi.ac.uk/pdbsum/1sa3 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1sa3 RCSB]</span>
| + | == Function == |
| - | }}
| + | [https://www.uniprot.org/uniprot/T2M1_MORSP T2M1_MORSP] Recognizes the double-stranded sequence CCGG and cleaves after C-1. |
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| - | '''An asymmetric complex of restriction endonuclease MspI on its palindromic DNA recognition site'''
| + | ==See Also== |
| - | | + | *[[Endonuclease 3D structures|Endonuclease 3D structures]] |
| - | | + | __TOC__ |
| - | ==Overview==
| + | </StructureSection> |
| - | Most well-known restriction endonucleases recognize palindromic DNA sequences and are classified as Type IIP. Due to the recognition and cleavage symmetry, Type IIP enzymes are usually found to act as homodimers in forming 2-fold symmetric enzyme-DNA complexes. Here we report an asymmetric complex of the Type IIP restriction enzyme MspI in complex with its cognate recognition sequence. Unlike any other Type IIP enzyme reported to date, an MspI monomer and not a dimer binds to a palindromic DNA sequence. The enzyme makes specific contacts with all 4 base pairs in the recognition sequence, by six direct and five water-mediated hydrogen bonds and numerous van der Waal contacts. This MspI-DNA structure represents the first example of asymmetric recognition of a palindromic DNA sequence by two different structural motifs in one polypeptide. A few possible pathways are discussed for MspI to cut both strands of DNA, either as a monomer or dimer.
| + | [[Category: Large Structures]] |
| - | | + | [[Category: Moraxella sp]] |
| - | ==About this Structure== | + | [[Category: Guo HC]] |
| - | 1SA3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Moraxella_sp. Moraxella sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SA3 OCA].
| + | [[Category: Kucera RB]] |
| - | | + | [[Category: Roberts RJ]] |
| - | ==Reference==
| + | [[Category: Xu QS]] |
| - | An asymmetric complex of restriction endonuclease MspI on its palindromic DNA recognition site., Xu QS, Kucera RB, Roberts RJ, Guo HC, Structure. 2004 Sep;12(9):1741-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15341737 15341737]
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| - | [[Category: Moraxella sp.]] | + | |
| - | [[Category: Single protein]] | + | |
| - | [[Category: Type II site-specific deoxyribonuclease]]
| + | |
| - | [[Category: Guo, H C.]] | + | |
| - | [[Category: Kucera, R B.]] | + | |
| - | [[Category: Roberts, R J.]] | + | |
| - | [[Category: Xu, Q S.]] | + | |
| - | [[Category: protein-dna complex]]
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| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:39:18 2008''
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