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1scs

From Proteopedia

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(New page: 200px<br /><applet load="1scs" size="450" color="white" frame="true" align="right" spinBox="true" caption="1scs, resolution 1.6&Aring;" /> '''HIGH-RESOLUTION STRUC...)
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[[Image:1scs.gif|left|200px]]<br /><applet load="1scs" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1scs, resolution 1.6&Aring;" />
 
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'''HIGH-RESOLUTION STRUCTURES OF SINGLE-METAL-SUBSTITUTED CONCANAVALIN A: THE CO,CA-PROTEIN AT 1.6 ANGSTROMS AND THE NI,CA-PROTEIN AT 2.0 ANGSTROMS'''<br />
 
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==Overview==
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==HIGH-RESOLUTION STRUCTURES OF SINGLE-METAL-SUBSTITUTED CONCANAVALIN A: THE CO,CA-PROTEIN AT 1.6 ANGSTROMS AND THE NI,CA-PROTEIN AT 2.0 ANGSTROMS==
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The molecular structures of cobalt- and nickel-substituted concanavalin A, have been refined at 1.6 and 2.0 A resolution, respectively. Both metal, derivatives crystallize in space group I222 with approximate cell, dimensions a = 89, b = 87 and c = 63 A and one monomer in the asymmetric, unit. The final R factor for Co-substituted concanavalin A is 17.8% for 29, 211 reflections with F &gt; 1.0sigma(F) between 8.0 and 1.6 A. For, Ni-substituted concanavalin A the final R factor is 15.9% for 16 128, reflections with F &gt; 1.0sigma(F) between 8.0 and 2.0 A resolution. Both, structures contain a transition-metal binding site and a calcium-binding, site but, unlike Cd-substituted concanavalin A, do not have a third, metal-binding site. The Co-substituted concanavalin A structure diffracts, to the highest resolution of any concanavalin A structure reported to, date. A comparison of the structures of Ni-, Co-, Cd-substituted and, native concanavalin A gives an indication of coordinate errors, which is a, useful baseline for comparisons with saccharide complexes of concanavalin, A described in other work. We also give a detailed account of multiple, conformations which were found for five side-chain residues.
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<StructureSection load='1scs' size='340' side='right'caption='[[1scs]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1scs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Canavalia_ensiformis Canavalia ensiformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SCS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SCS FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1scs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1scs OCA], [https://pdbe.org/1scs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1scs RCSB], [https://www.ebi.ac.uk/pdbsum/1scs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1scs ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CONA_CANEN CONA_CANEN] D-mannose specific lectin.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sc/1scs_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1scs ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1SCS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Canavalia_ensiformis Canavalia ensiformis] with CO and CA as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SCS OCA].
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*[[Concanavalin 3D structures|Concanavalin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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High-resolution structures of single-metal-substituted concanavalin A: the Co,Ca-protein at 1.6 A and the Ni,Ca-protein at 2.0 A., Emmerich C, Helliwell JR, Redshaw M, Naismith JH, Harrop SJ, Raftery J, Kalb AJ, Yariv J, Dauter Z, Wilson KS, Acta Crystallogr D Biol Crystallogr. 1994 Sep 1;50(Pt 5):749-56. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15299372 15299372]
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[[Category: Canavalia ensiformis]]
[[Category: Canavalia ensiformis]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Dauter, Z.]]
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[[Category: Dauter Z]]
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[[Category: Emmerich, C.]]
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[[Category: Emmerich C]]
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[[Category: Harrop, S.J.]]
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[[Category: Harrop SJ]]
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[[Category: Helliwell, J.R.]]
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[[Category: Helliwell JR]]
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[[Category: Kalb, A.J.]]
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[[Category: Kalb AJ]]
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[[Category: Naismith, J.H.]]
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[[Category: Naismith JH]]
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[[Category: Raftery, J.]]
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[[Category: Raftery J]]
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[[Category: Redshaw, M.]]
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[[Category: Redshaw M]]
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[[Category: Wilson, K.S.]]
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[[Category: Wilson KS]]
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[[Category: Yariv, J.]]
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[[Category: Yariv J]]
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[[Category: CA]]
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[[Category: CO]]
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[[Category: lectin(agglutinin)]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:16:56 2007''
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Current revision

HIGH-RESOLUTION STRUCTURES OF SINGLE-METAL-SUBSTITUTED CONCANAVALIN A: THE CO,CA-PROTEIN AT 1.6 ANGSTROMS AND THE NI,CA-PROTEIN AT 2.0 ANGSTROMS

PDB ID 1scs

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