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| - | [[Image:1skm.gif|left|200px]] | |
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| - | {{Structure
| + | ==HhaI methyltransferase in complex with DNA containing an abasic south carbocyclic sugar at its target site== |
| - | |PDB= 1skm |SIZE=350|CAPTION= <scene name='initialview01'>1skm</scene>, resolution 2.2Å
| + | <StructureSection load='1skm' size='340' side='right'caption='[[1skm]], [[Resolution|resolution]] 2.20Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=HCX:(SOUTH)+BICYCLO[3.1.0]HEXANE'>HCX</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>
| + | <table><tr><td colspan='2'>[[1skm]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_haemolyticus Haemophilus haemolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SKM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SKM FirstGlance]. <br> |
| - | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_(cytosine-5-)-methyltransferase DNA (cytosine-5-)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.37 2.1.1.37] </span>
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> |
| - | |GENE= HHAIM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=726 Haemophilus haemolyticus])
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HCX:(SOUTH)+BICYCLO[3.1.0]HEXANE'>HCX</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr> |
| - | |DOMAIN=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1skm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1skm OCA], [https://pdbe.org/1skm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1skm RCSB], [https://www.ebi.ac.uk/pdbsum/1skm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1skm ProSAT]</span></td></tr> |
| - | |RELATEDENTRY=[[1hmy|1HMY]], [[1mht|1MHT]], [[1fjx|1FJX]], [[9mht|9MHT]]
| + | </table> |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1skm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1skm OCA], [http://www.ebi.ac.uk/pdbsum/1skm PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1skm RCSB]</span>
| + | == Function == |
| - | }}
| + | [https://www.uniprot.org/uniprot/MTH1_HAEPH MTH1_HAEPH] This methylase recognizes the double-stranded sequence GCGC, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the HhaI endonuclease. |
| | + | == Evolutionary Conservation == |
| | + | [[Image:Consurf_key_small.gif|200px|right]] |
| | + | Check<jmol> |
| | + | <jmolCheckbox> |
| | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sk/1skm_consurf.spt"</scriptWhenChecked> |
| | + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| | + | <text>to colour the structure by Evolutionary Conservation</text> |
| | + | </jmolCheckbox> |
| | + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1skm ConSurf]. |
| | + | <div style="clear:both"></div> |
| | | | |
| - | '''HhaI methyltransferase in complex with DNA containing an abasic south carbocyclic sugar at its target site'''
| + | ==See Also== |
| - | | + | *[[DNA methyltransferase 3D structures|DNA methyltransferase 3D structures]] |
| - | | + | __TOC__ |
| - | ==Overview== | + | </StructureSection> |
| - | Rotation of a DNA or RNA nucleotide out of the double helix and into a protein pocket ('base flipping') is a mechanistic feature common to some DNA/RNA-binding proteins. Here, we report the structure of HhaI methyltransferase in complex with DNA containing a south-constrained abasic carbocyclic sugar at the target site in the presence of the methyl donor byproduct AdoHcy. Unexpectedly, the locked south pseudosugar appears to be trapped in the middle of the flipping pathway via the DNA major groove, held in place primarily through Van der Waals contacts with a set of invariant amino acids. Molecular dynamics simulations indicate that the structural stabilization observed with the south-constrained pseudosugar will not occur with a north-constrained pseudosugar, which explains its lowered binding affinity. Moreover, comparison of structural transitions of the sugar and phosphodiester backbone observed during computational studies of base flipping in the M.HhaI-DNA-AdoHcy ternary complex indicate that the south-constrained pseudosugar induces a conformation on the phosphodiester backbone that corresponds to that of a discrete intermediate of the base-flipping pathway. As previous crystal structures of M.HhaI ternary complex with DNA displayed the flipped sugar moiety in the antipodal north conformation, we suggest that conversion of the sugar pucker from south to north beyond the middle of the pathway is an essential part of the mechanism through which flipping must proceed to reach its final destination. We also discuss the possibility of the south-constrained pseudosugar mimicking a transition state in the phosphodiester and sugar moieties that occurs during DNA base flipping in the presence of M.HhaI.
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| - | | + | |
| - | ==About this Structure==
| + | |
| - | 1SKM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Haemophilus_haemolyticus Haemophilus haemolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SKM OCA].
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| - | | + | |
| - | ==Reference==
| + | |
| - | Caught in the act: visualization of an intermediate in the DNA base-flipping pathway induced by HhaI methyltransferase., Horton JR, Ratner G, Banavali NK, Huang N, Choi Y, Maier MA, Marquez VE, MacKerell AD Jr, Cheng X, Nucleic Acids Res. 2004 Jul 23;32(13):3877-86. Print 2004. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15273274 15273274]
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| - | [[Category: DNA (cytosine-5-)-methyltransferase]]
| + | |
| | [[Category: Haemophilus haemolyticus]] | | [[Category: Haemophilus haemolyticus]] |
| - | [[Category: Single protein]] | + | [[Category: Large Structures]] |
| - | [[Category: Banavali, N.]] | + | [[Category: Banavali N]] |
| - | [[Category: Cheng, X.]] | + | [[Category: Cheng X]] |
| - | [[Category: Horton, J R.]] | + | [[Category: Horton JR]] |
| - | [[Category: Huang, N.]] | + | [[Category: Huang N]] |
| - | [[Category: MacKerell, A D.]] | + | [[Category: MacKerell AD]] |
| - | [[Category: Marquez, V E.]] | + | [[Category: Marquez VE]] |
| - | [[Category: Ratner, G.]] | + | [[Category: Ratner G]] |
| - | [[Category: protein-dna complex containing constrained abasic unnatural base]]
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:43:20 2008''
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