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1sqc

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[[Image:1sqc.jpg|left|200px]]<br /><applet load="1sqc" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1sqc, resolution 2.85&Aring;" />
 
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'''SQUALENE-HOPENE-CYCLASE FROM ALICYCLOBACILLUS ACIDOCALDARIUS'''<br />
 
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==Overview==
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==SQUALENE-HOPENE-CYCLASE FROM ALICYCLOBACILLUS ACIDOCALDARIUS==
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The crystal structure of squalene-hopene cyclase from Alicyclobacillus, acidocaldarius was determined at 2.9 angstrom resolution. The mechanism, and sequence of this cyclase are closely related to those of, 2,3-oxidosqualene cyclases that catalyze the cyclization step in, cholesterol biosynthesis. The structure reveals a membrane protein with, membrane-binding characteristics similar to those of prostaglandin-H2, synthase, the only other reported protein of this type. The active site of, the enzyme is located in a large central cavity that is of suitable size, to bind squalene in its required conformation and that is lined by, aromatic residues. The structure supports a mechanism in which the acid, starting the reaction by protonating a carbon-carbon double bond is an, aspartate that is coupled to a histidine. Numerous surface alpha helices, are connected by characteristic QW-motifs (Q is glutamine and W is, tryptophan) that tighten the protein structure, possibly for absorbing the, reaction energy without structural damage.
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<StructureSection load='1sqc' size='340' side='right'caption='[[1sqc]], [[Resolution|resolution]] 2.85&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1sqc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Alicyclobacillus_acidocaldarius Alicyclobacillus acidocaldarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SQC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SQC FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.85&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sqc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sqc OCA], [https://pdbe.org/1sqc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sqc RCSB], [https://www.ebi.ac.uk/pdbsum/1sqc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sqc ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SQHC_ALIAD SQHC_ALIAD] Catalyzes the cyclization of squalene into hopene.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sq/1sqc_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sqc ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1SQC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Alicyclobacillus_acidocaldarius Alicyclobacillus acidocaldarius] with LDA as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=LDA:The Active Site Is Located In A Large Central Cavity, As ...'>LDA</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SQC OCA].
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*[[Squalene-hopene cyclase|Squalene-hopene cyclase]]
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__TOC__
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==Reference==
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</StructureSection>
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Structure and function of a squalene cyclase., Wendt KU, Poralla K, Schulz GE, Science. 1997 Sep 19;277(5333):1811-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9295270 9295270]
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[[Category: Alicyclobacillus acidocaldarius]]
[[Category: Alicyclobacillus acidocaldarius]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Schulz, G.E.]]
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[[Category: Schulz GE]]
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[[Category: Wendt, K.U.]]
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[[Category: Wendt KU]]
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[[Category: LDA]]
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[[Category: 29-ene) and diplopterol (hopane-22-ol)]]
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[[Category: isomerase]]
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[[Category: membrane protein]]
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[[Category: squalene to hopene (hop-22]]
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[[Category: terpenoid metabolism]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 18:00:56 2007''
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Current revision

SQUALENE-HOPENE-CYCLASE FROM ALICYCLOBACILLUS ACIDOCALDARIUS

PDB ID 1sqc

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