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1swi

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==GCN4-LEUCINE ZIPPER CORE MUTANT AS N16A COMPLEXED WITH BENZENE==
==GCN4-LEUCINE ZIPPER CORE MUTANT AS N16A COMPLEXED WITH BENZENE==
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<StructureSection load='1swi' size='340' side='right' caption='[[1swi]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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<StructureSection load='1swi' size='340' side='right'caption='[[1swi]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[1swi]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SWI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SWI FirstGlance]. <br>
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<table><tr><td colspan='2'>[[1swi]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SWI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SWI FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BNZ:BENZENE'>BNZ</scene><br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1swi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1swi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1swi RCSB], [http://www.ebi.ac.uk/pdbsum/1swi PDBsum]</span></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BNZ:BENZENE'>BNZ</scene></td></tr>
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<table>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1swi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1swi OCA], [https://pdbe.org/1swi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1swi RCSB], [https://www.ebi.ac.uk/pdbsum/1swi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1swi ProSAT]</span></td></tr>
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<div style="background-color:#fffaf0;">
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</table>
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== Publication Abstract from PubMed ==
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== Function ==
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Controversy remains about the role of core side-chain packing in specifying protein structure. To investigate the influence of core packing on the oligomeric structure of a coiled coil, we engineered a GCN4 leucine zipper mutant that switches from two to three strands upon binding the hydrophobic ligands cyclohexane and benzene. In solution these ligands increased the apparent thermal stability and the oligomerization order of the mutant leucine zipper. The crystal structure of the peptide-benzene complex shows a single benzene molecule bound at the engineered site in the core of the trimer. These results indicate that coiled coils are well-suited to function as molecular switches and emphasize that core packing is an important determinant of oligomerization specificity.
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[https://www.uniprot.org/uniprot/GCN4_YEAST GCN4_YEAST] Is a transcription factor that is responsible for the activation of more than 30 genes required for amino acid or for purine biosynthesis in response to amino acid or purine starvation. Binds and recognize the DNA sequence: 5'-TGA[CG]TCA-3'.
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An engineered allosteric switch in leucine-zipper oligomerization.,Gonzalez L Jr, Plecs JJ, Alber T Nat Struct Biol. 1996 Jun;3(6):510-5. PMID:8646536<ref>PMID:8646536</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==See Also==
==See Also==
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*[[Gcn4|Gcn4]]
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*[[Gcn4 3D Structures|Gcn4 3D Structures]]
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== References ==
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*[[Gnc4 3D Structures|Gnc4 3D Structures]]
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Alber, T.]]
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[[Category: Alber T]]
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[[Category: Gonzalez, L.]]
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[[Category: Gonzalez L]]
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[[Category: Plecs, J.]]
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[[Category: Plecs J]]
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[[Category: Leucine zipper]]
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Current revision

GCN4-LEUCINE ZIPPER CORE MUTANT AS N16A COMPLEXED WITH BENZENE

PDB ID 1swi

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