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1szi

From Proteopedia

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[[Image:1szi.gif|left|200px]]<br /><applet load="1szi" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1szi, resolution 2.80&Aring;" />
 
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'''Crystal Structure of the C-terminus of TIP47'''<br />
 
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==Overview==
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==Crystal Structure of the C-terminus of TIP47==
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The perilipin/ADRP/TIP47 (PAT) proteins localize to the surface of intracellular neutral lipid droplets. Perilipin is essential for lipid storage and hormone regulated lipolysis in adipocytes, and perilipin null mice exhibit a dramatic reduction in adipocyte lipid stores. A significant fraction of the approximately 200 amino acid N-terminal region of the PAT proteins consists of 11-mer helical repeats that are also found in apolipoproteins and other lipid-associated proteins. The C-terminal 60% of TIP47, a representative PAT protein, comprises a monomeric and independently folded unit. The crystal structure of the C-terminal portion of TIP47 was determined and refined at 2.8 A resolution. The structure consists of an alpha/beta domain of novel topology and a four-helix bundle resembling the LDL receptor binding domain of apolipoprotein E. The structure suggests an analogy between PAT proteins and apolipoproteins in which helical repeats interact with lipid while the ordered C-terminal region is involved in protein:protein interactions.
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<StructureSection load='1szi' size='340' side='right'caption='[[1szi]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1szi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SZI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SZI FirstGlance]. <br>
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1SZI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SZI OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1szi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1szi OCA], [https://pdbe.org/1szi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1szi RCSB], [https://www.ebi.ac.uk/pdbsum/1szi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1szi ProSAT]</span></td></tr>
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==Reference==
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</table>
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Structure of a lipid droplet protein; the PAT family member TIP47., Hickenbottom SJ, Kimmel AR, Londos C, Hurley JH, Structure. 2004 Jul;12(7):1199-207. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15242596 15242596]
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== Function ==
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[https://www.uniprot.org/uniprot/PLIN3_MOUSE PLIN3_MOUSE] Required for the transport of mannose 6-phosphate receptors (MPR) from endosomes to the trans-Golgi network (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sz/1szi_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1szi ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Single protein]]
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[[Category: Hickenbottom SJ]]
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[[Category: Hickenbottom, S J.]]
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[[Category: Hurley JH]]
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[[Category: Hurley, J H.]]
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[[Category: Kimmel AR]]
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[[Category: Kimmel, A R.]]
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[[Category: Londos C]]
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[[Category: Londos, C.]]
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[[Category: 4-helix bundle]]
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[[Category: alpha/beta domain]]
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[[Category: pat protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:07:51 2008''
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Current revision

Crystal Structure of the C-terminus of TIP47

PDB ID 1szi

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