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1t00

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[[Image:1t00.gif|left|200px]]
 
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{{Structure
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==The structure of thioredoxin from S. coelicolor==
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|PDB= 1t00 |SIZE=350|CAPTION= <scene name='initialview01'>1t00</scene>, resolution 1.51&Aring;
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<StructureSection load='1t00' size='340' side='right'caption='[[1t00]], [[Resolution|resolution]] 1.51&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1t00]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_coelicolor_A3(2) Streptomyces coelicolor A3(2)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T00 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T00 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.51&#8491;</td></tr>
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|GENE= TRXA, SCO3889, SCH24.11C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 Streptomyces coelicolor])
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t00 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t00 OCA], [https://pdbe.org/1t00 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t00 RCSB], [https://www.ebi.ac.uk/pdbsum/1t00 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t00 ProSAT]</span></td></tr>
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}}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/THIO1_STRCO THIO1_STRCO] Component of the thioredoxin-thioredoxin reductase system. Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Stimulates complex formation between sigma factor SigR and its cognate anti-sigma factor RsrA probably by reducing RsrA.<ref>PMID:10428967</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t0/1t00_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t00 ConSurf].
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<div style="clear:both"></div>
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'''The structure of thioredoxin from S. coelicolor'''
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==See Also==
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*[[Thioredoxin 3D structures|Thioredoxin 3D structures]]
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== References ==
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==Overview==
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<references/>
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Thioredoxins are ubiquitous proteins that serve as reducing agents and general protein disulfide reductases. In turn, they are reduced by electrons obtained from the NADPH-containing thioredoxin reductase. Thioredoxins have been isolated and characterized from a large number of organisms. The Gram-positive bacterium Streptomyces coelicolor contains three thioredoxins that are involved in unknown biological processes. trxA from S. coelicolor was cloned and expressed in Escherichia coli and the protein purified and crystallized using the hanging-drop method of vapour diffusion. The crystal structure of thioredoxin A has been determined at 1.5 A resolution using a synchrotron-radiation source. The protein reveals a thioredoxin-like fold with a typical CXXC active site. The crystal exhibits the symmetry of space group P2(1)2(1)2, with unit-cell parameters a = 43.6, b = 71.8, c = 33.2 A.
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1T00 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T00 OCA].
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[[Category: Stefankova P]]
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==Reference==
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Expression, purification and X-ray crystallographic analysis of thioredoxin from Streptomyces coelicolor., Stefankova P, Maderova J, Barak I, Kollarova M, Otwinowski Z, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Feb 1;61(Pt, 2):164-8. Epub 2005 Jan 8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16510983 16510983]
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[[Category: Single protein]]
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[[Category: Streptomyces coelicolor]]
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[[Category: Stefankova, P.]]
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[[Category: multifunction macromolecule]]
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[[Category: redox regulation]]
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[[Category: s. coelicolor]]
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[[Category: structure]]
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[[Category: thioredoxin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:11:49 2008''
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Current revision

The structure of thioredoxin from S. coelicolor

PDB ID 1t00

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