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1t11

From Proteopedia

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(New page: 200px<br /><applet load="1t11" size="450" color="white" frame="true" align="right" spinBox="true" caption="1t11, resolution 2.50&Aring;" /> '''Trigger Factor'''<br...)
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[[Image:1t11.gif|left|200px]]<br /><applet load="1t11" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1t11, resolution 2.50&Aring;" />
 
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'''Trigger Factor'''<br />
 
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==Overview==
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==Trigger Factor==
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Trigger factor is a molecular chaperone that is present in all species of, eubacteria. It binds to the ribosomal 50S subunit near the translation, exit tunnel and is thought to be the first protein to interact with, nascent polypeptides emerging from the ribosome. The chaperone has a, peptidyl-prolyl cis-trans isomerase (PPIase) activity that catalyzes the, rate-limiting proline isomerization in the protein-folding process. We, have determined the crystal structure of nearly full-length trigger factor, from Vibrio cholerae by x-ray crystallography at 2.5-A resolution. The, structure is composed of two trigger-factor molecules related by a, noncrystallographic two-fold symmetry axis. The monomer has an elongated, shape and is folded into three domains: an N-terminal domain I that binds, to the ribosome, a central domain II that contains PPIase activity, and a, C-terminal domain III. The active site of the PPIase domain is occupied by, a loop from domain III, suggesting that the PPIase activity of the protein, could be regulated. The dimer interface is formed between domains I and, III and contains residues of mixed properties. Further implications about, dimerization, ribosome binding, and other functions of trigger factor are, discussed.
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<StructureSection load='1t11' size='340' side='right'caption='[[1t11]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1t11]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T11 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T11 FirstGlance]. <br>
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1T11 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1T11 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t11 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t11 OCA], [https://pdbe.org/1t11 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t11 RCSB], [https://www.ebi.ac.uk/pdbsum/1t11 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t11 ProSAT]</span></td></tr>
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==Reference==
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</table>
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The crystal structure of ribosomal chaperone trigger factor from Vibrio cholerae., Ludlam AV, Moore BA, Xu Z, Proc Natl Acad Sci U S A. 2004 Sep 14;101(37):13436-41. Epub 2004 Sep 7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15353602 15353602]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/TIG_VIBCH TIG_VIBCH] Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation. Functions as a peptidyl-prolyl cis-trans isomerase (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t1/1t11_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t11 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Vibrio cholerae]]
[[Category: Vibrio cholerae]]
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[[Category: Ludlam, A.V.]]
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[[Category: Ludlam AV]]
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[[Category: Moore, B.A.]]
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[[Category: Moore BA]]
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[[Category: Xu, Z.]]
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[[Category: Xu Z]]
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[[Category: four-helix-bundle]]
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[[Category: helix-turn-helix]]
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[[Category: ppiase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:54:35 2007''
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Current revision

Trigger Factor

PDB ID 1t11

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