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1t27

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[[Image:1t27.jpg|left|200px]]<br /><applet load="1t27" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1t27, resolution 2.20&Aring;" />
 
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'''THE STRUCTURE OF PITP COMPLEXED TO PHOSPHATIDYLCHOLINE'''<br />
 
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==Overview==
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==THE STRUCTURE OF PITP COMPLEXED TO PHOSPHATIDYLCHOLINE==
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Eukaryotic phosphatidylinositol transfer protein is a ubiquitous multifunctional protein that transports phospholipids between membrane surfaces and participates in cellular phospholipid metabolism during signal transduction and vesicular trafficking. The three-dimensional structure of the alpha-isoform of rat phosphatidylinositol transfer protein complexed with one molecule of phosphatidylcholine, one of its physiological ligands, has been determined to 2.2 A resolution by x-ray diffraction techniques. A single beta-sheet and several long alpha-helices define an enclosed internal cavity in which a single molecule of the phospholipid is accommodated with its polar head group in the center of the protein and fatty acyl chains projected toward the surface. Other structural features suggest mechanisms by which cytosolic phosphatidylinositol transfer protein interacts with membranes for lipid exchange and associates with a variety of lipid and protein kinases.
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<StructureSection load='1t27' size='340' side='right'caption='[[1t27]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1t27]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1fvz 1fvz]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T27 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T27 FirstGlance]. <br>
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1T27 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with <scene name='pdbligand=PCW:'>PCW</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. This structure supersedes the now removed PDB entry 1FVZ. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T27 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PCW:1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE'>PCW</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t27 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t27 OCA], [https://pdbe.org/1t27 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t27 RCSB], [https://www.ebi.ac.uk/pdbsum/1t27 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t27 ProSAT]</span></td></tr>
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Structure of a multifunctional protein. Mammalian phosphatidylinositol transfer protein complexed with phosphatidylcholine., Yoder MD, Thomas LM, Tremblay JM, Oliver RL, Yarbrough LR, Helmkamp GM Jr, J Biol Chem. 2001 Mar 23;276(12):9246-52. Epub 2000 Dec 4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11104777 11104777]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PIPNA_RAT PIPNA_RAT] Catalyzes the transfer of PtdIns and phosphatidylcholine between membranes.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t2/1t27_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t27 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
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[[Category: Single protein]]
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[[Category: Helmkamp Jr GM]]
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[[Category: Jr., G M.Helmkamp.]]
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[[Category: Oliver RL]]
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[[Category: Oliver, R L.]]
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[[Category: Thomas LM]]
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[[Category: Thomas, L M.]]
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[[Category: Tremblay JM]]
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[[Category: Tremblay, J M.]]
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[[Category: Yarbrough LR]]
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[[Category: Yarbrough, L R.]]
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[[Category: Yoder MD]]
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[[Category: Yoder, M D.]]
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[[Category: PCW]]
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[[Category: lipid binding protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:09:08 2008''
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Current revision

THE STRUCTURE OF PITP COMPLEXED TO PHOSPHATIDYLCHOLINE

PDB ID 1t27

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