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1t5s

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[[Image:1t5s.gif|left|200px]]
 
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{{Structure
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==Structure of the (SR)Ca2+-ATPase Ca2-E1-AMPPCP form==
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|PDB= 1t5s |SIZE=350|CAPTION= <scene name='initialview01'>1t5s</scene>, resolution 2.60&Aring;
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<StructureSection load='1t5s' size='340' side='right'caption='[[1t5s]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC+ACID+ADENYLATE+ESTER'>ACP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
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<table><tr><td colspan='2'>[[1t5s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T5S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T5S FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Calcium-transporting_ATPase Calcium-transporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.8 3.6.3.8] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC+ACID+ADENYLATE+ESTER'>ACP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t5s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t5s OCA], [https://pdbe.org/1t5s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t5s RCSB], [https://www.ebi.ac.uk/pdbsum/1t5s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t5s ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1t5t|1T5T]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t5s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t5s OCA], [http://www.ebi.ac.uk/pdbsum/1t5s PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1t5s RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/AT2A1_RABIT AT2A1_RABIT] This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t5/1t5s_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t5s ConSurf].
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<div style="clear:both"></div>
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'''Structure of the (SR)Ca2+-ATPase Ca2-E1-AMPPCP form'''
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==See Also==
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*[[ATPase 3D structures|ATPase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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A tight coupling between adenosine triphosphate (ATP) hydrolysis and vectorial ion transport has to be maintained by ATP-consuming ion pumps. We report two crystal structures of Ca2+-bound sarco(endo)plasmic reticulum Ca2+-adenosine triphosphatase (SERCA) at 2.6 and 2.9 angstrom resolution in complex with (i) a nonhydrolyzable ATP analog [adenosine (beta-gamma methylene)-triphosphate] and (ii) adenosine diphosphate plus aluminum fluoride. SERCA reacts with ATP by an associative mechanism mediated by two Mg2+ ions to form an aspartyl-phosphorylated intermediate state (Ca2-E1 approximately P). The conformational changes that accompany the reaction with ATP pull the transmembrane helices 1 and 2 and close a cytosolic entrance for Ca2+, thereby preventing backflow before Ca2+ is released on the other side of the membrane.
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[[Category: Large Structures]]
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==About this Structure==
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1T5S is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T5S OCA].
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==Reference==
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Phosphoryl transfer and calcium ion occlusion in the calcium pump., Sorensen TL, Moller JV, Nissen P, Science. 2004 Jun 11;304(5677):1672-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15192230 15192230]
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[[Category: Calcium-transporting ATPase]]
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[[Category: Oryctolagus cuniculus]]
[[Category: Oryctolagus cuniculus]]
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[[Category: Single protein]]
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[[Category: Moller JV]]
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[[Category: Moller, J V.]]
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[[Category: Nissen P]]
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[[Category: Nissen, P.]]
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[[Category: Sorensen TL-M]]
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[[Category: Sorensen, T L.M.]]
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[[Category: calcium pump]]
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[[Category: catalytic mechanism]]
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[[Category: domain movement]]
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[[Category: membrane protein]]
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[[Category: nonhydrolyzable atp analog]]
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[[Category: nucleotide binding]]
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[[Category: phosphorylation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:51:40 2008''
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Current revision

Structure of the (SR)Ca2+-ATPase Ca2-E1-AMPPCP form

PDB ID 1t5s

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