1t5t

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(New page: 200px<br /><applet load="1t5t" size="450" color="white" frame="true" align="right" spinBox="true" caption="1t5t, resolution 2.90&Aring;" /> '''Structure of the (SR...)
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[[Image:1t5t.gif|left|200px]]<br /><applet load="1t5t" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1t5t, resolution 2.90&Aring;" />
 
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'''Structure of the (SR)Ca2+-ATPase Ca2-E1-ADP:AlF4- form'''<br />
 
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==Overview==
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==Structure of the (SR)Ca2+-ATPase Ca2-E1-ADP:AlF4- form==
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A tight coupling between adenosine triphosphate (ATP) hydrolysis and, vectorial ion transport has to be maintained by ATP-consuming ion pumps., We report two crystal structures of Ca2+-bound sarco(endo)plasmic, reticulum Ca2+-adenosine triphosphatase (SERCA) at 2.6 and 2.9 angstrom, resolution in complex with (i) a nonhydrolyzable ATP analog [adenosine, (beta-gamma methylene)-triphosphate] and (ii) adenosine diphosphate plus, aluminum fluoride. SERCA reacts with ATP by an associative mechanism, mediated by two Mg2+ ions to form an aspartyl-phosphorylated intermediate, state (Ca2-E1 approximately P). The conformational changes that accompany, the reaction with ATP pull the transmembrane helices 1 and 2 and close a, cytosolic entrance for Ca2+, thereby preventing backflow before Ca2+ is, released on the other side of the membrane.
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<StructureSection load='1t5t' size='340' side='right'caption='[[1t5t]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1t5t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T5T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T5T FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ALF:TETRAFLUOROALUMINATE+ION'>ALF</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t5t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t5t OCA], [https://pdbe.org/1t5t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t5t RCSB], [https://www.ebi.ac.uk/pdbsum/1t5t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t5t ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AT2A1_RABIT AT2A1_RABIT] This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t5/1t5t_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t5t ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1T5T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus] with ALF, CA, MG, K and ADP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Calcium-transporting_ATPase Calcium-transporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.8 3.6.3.8] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1T5T OCA].
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*[[ATPase 3D structures|ATPase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Phosphoryl transfer and calcium ion occlusion in the calcium pump., Sorensen TL, Moller JV, Nissen P, Science. 2004 Jun 11;304(5677):1672-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15192230 15192230]
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[[Category: Large Structures]]
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[[Category: Calcium-transporting ATPase]]
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[[Category: Oryctolagus cuniculus]]
[[Category: Oryctolagus cuniculus]]
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[[Category: Single protein]]
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[[Category: Moller JV]]
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[[Category: Moller, J.V.]]
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[[Category: Nissen P]]
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[[Category: Nissen, P.]]
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[[Category: Sorensen TL-M]]
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[[Category: Sorensen, T.L.M.]]
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[[Category: ADP]]
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[[Category: ALF]]
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[[Category: CA]]
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[[Category: K]]
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[[Category: MG]]
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[[Category: calcium pump]]
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[[Category: catalytic mechanism]]
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[[Category: membrane protein]]
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[[Category: occlusion]]
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[[Category: phosphorylation]]
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[[Category: transition state]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 03:00:43 2007''
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Current revision

Structure of the (SR)Ca2+-ATPase Ca2-E1-ADP:AlF4- form

PDB ID 1t5t

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