1thm

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[[Image:1thm.gif|left|200px]]
 
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==CRYSTAL STRUCTURE OF THERMITASE AT 1.4 ANGSTROMS RESOLUTION==
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The line below this paragraph, containing "STRUCTURE_1thm", creates the "Structure Box" on the page.
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<StructureSection load='1thm' size='340' side='right'caption='[[1thm]], [[Resolution|resolution]] 1.37&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1thm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermoactinomyces_vulgaris Thermoactinomyces vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1THM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1THM FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.37&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_1thm| PDB=1thm | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1thm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1thm OCA], [https://pdbe.org/1thm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1thm RCSB], [https://www.ebi.ac.uk/pdbsum/1thm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1thm ProSAT]</span></td></tr>
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</table>
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'''CRYSTAL STRUCTURE OF THERMITASE AT 1.4 ANGSTROMS RESOLUTION'''
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== Function ==
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[https://www.uniprot.org/uniprot/THET_THEVU THET_THEVU]
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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The crystal structure of thermitase, a subtilisin-type serine proteinase from Thermoactinomyces vulgaris, was determined by X-ray diffraction at 1.4 A resolution. The structure was solved by a combination of molecular and isomorphous replacement. The starting model was that of subtilisin BPN' from the Protein Data Bank, determined at 2.5 A resolution. The high-resolution refinement was based on data collected using synchrotron radiation with a Fuji image plate as detector. The model of thermitase refined to a conventional R factor of 14.9% and contains 1997 protein atoms, 182 water molecules and two Ca ions. The tertiary structure of thermitase is similar to that of the other subtilisins although there are some significant differences in detail. Comparison with subtilisin BPN' revealed two major structural differences. The N-terminal region in thermitase, which is absent in subtilisin BPN', forms a number of contacts with the tight Ca2+ binding site and indeed provides the very tight binding of the Ca ion. In thermitase the loop of residues 60 to 65 forms an additional (10) beta-strand of the central beta-sheet and the second Ca2+ binding site that has no equivalent in the subtilisin BPN' structure. The observed differences in the Ca2+ binding and the increased number of ionic and aromatic interactions in thermitase are likely sources of the enhanced stability of thermitase.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/th/1thm_consurf.spt"</scriptWhenChecked>
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1THM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermoactinomyces_vulgaris Thermoactinomyces vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1THM OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Crystal structure of thermitase at 1.4 A resolution., Teplyakov AV, Kuranova IP, Harutyunyan EH, Vainshtein BK, Frommel C, Hohne WE, Wilson KS, J Mol Biol. 1990 Jul 5;214(1):261-79. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/2196375 2196375]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1thm ConSurf].
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[[Category: Single protein]]
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<div style="clear:both"></div>
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[[Category: Thermitase]]
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Thermoactinomyces vulgaris]]
[[Category: Thermoactinomyces vulgaris]]
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[[Category: Harutyunyan, E H.]]
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[[Category: Harutyunyan EH]]
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[[Category: Kuranova, I P.]]
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[[Category: Kuranova IP]]
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[[Category: Teplyakov, A V.]]
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[[Category: Teplyakov AV]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:57:25 2008''
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Current revision

CRYSTAL STRUCTURE OF THERMITASE AT 1.4 ANGSTROMS RESOLUTION

PDB ID 1thm

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