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1tlw

From Proteopedia

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(New page: 200px<br /><applet load="1tlw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tlw, resolution 3.10&Aring;" /> '''Tsx structure comple...)
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[[Image:1tlw.jpg|left|200px]]<br /><applet load="1tlw" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1tlw, resolution 3.10&Aring;" />
 
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'''Tsx structure complexed with thymidine'''<br />
 
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==Overview==
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==Tsx structure complexed with thymidine==
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Tsx is a nucleoside-specific outer membrane (OM) transporter of, Gram-negative bacteria. We present crystal structures of Escherichia coli, Tsx in the absence and presence of nucleosides. These structures provide a, mechanism for nucleoside transport across the bacterial OM. Tsx forms a, monomeric, 12-stranded beta-barrel with a long and narrow channel spanning, the outer membrane. The channel, which is shaped like a keyhole, contains, several distinct nucleoside-binding sites, two of which are well defined., The base moiety of the nucleoside is located in the narrow part of the, keyhole, while the sugar occupies the wider opening. Pairs of aromatic, residues and flanking ionizable residues are involved in nucleoside, binding. Nucleoside transport presumably occurs by diffusion from one, binding site to the next.
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<StructureSection load='1tlw' size='340' side='right'caption='[[1tlw]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1tlw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TLW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TLW FirstGlance]. <br>
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1TLW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with THM as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TLW OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=THM:THYMIDINE'>THM</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tlw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tlw OCA], [https://pdbe.org/1tlw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tlw RCSB], [https://www.ebi.ac.uk/pdbsum/1tlw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tlw ProSAT]</span></td></tr>
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Crystal structure of the bacterial nucleoside transporter Tsx., Ye J, van den Berg B, EMBO J. 2004 Aug 18;23(16):3187-95. Epub 2004 Jul 22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15272310 15272310]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TSX_ECOLI TSX_ECOLI] Constitutes the receptor for colicin K and phage T6, and functions as substrate-specific channel for nucleosides and deoxynucleosides.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tl/1tlw_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tlw ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Berg, B.van.den.]]
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[[Category: Ye J]]
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[[Category: Ye, J.]]
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[[Category: Van den Berg B]]
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[[Category: THM]]
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[[Category: beta barrel]]
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[[Category: nucleoside transporter]]
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[[Category: thymidine]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 03:23:35 2007''
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Current revision

Tsx structure complexed with thymidine

PDB ID 1tlw

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