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1tuw

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[[Image:1tuw.jpg|left|200px]]<br /><applet load="1tuw" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1tuw, resolution 1.9&Aring;" />
 
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'''Structural and Functional Analysis of Tetracenomycin F2 Cyclase from Streptomyces glaucescens: A Type-II Polyketide Cyclase'''<br />
 
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==Overview==
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==Structural and Functional Analysis of Tetracenomycin F2 Cyclase from Streptomyces glaucescens: A Type-II Polyketide Cyclase==
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Tetracenomycin F2 cyclase (tcmI gene product), catalyzes an aromatic rearrangement in the biosynthetic pathway for tetracenomycin C in Streptomyces glaucescens. The x-ray structure of this small enzyme has been determined to 1.9-A resolution together with an analysis of site-directed mutants of potential catalytic residues. The protein exhibits a dimeric betaalphabeta ferredoxin-like fold that utilizes strand swapping between subunits in its assembly. The fold is dominated by four strands of antiparallel sheet and a layer of alpha-helices, which creates a cavity that is proposed to be the active site. This type of secondary structural arrangement has been previously observed in polyketide monooxygenases and suggests an evolutionary relationship between enzymes that catalyze adjacent steps in these biosynthetic pathways. Mutational analysis of all of the obvious catalytic bases within the active site suggests that the enzyme functions to steer the chemical outcome of the cyclization rather than providing a specific catalytic group. Together, the structure and functional analysis provide insight into the structural framework necessary to perform the complex rearrangements catalyzed by this class of polyketide cyclases.
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<StructureSection load='1tuw' size='340' side='right'caption='[[1tuw]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1tuw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_glaucescens Streptomyces glaucescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TUW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TUW FirstGlance]. <br>
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1TUW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_glaucescens Streptomyces glaucescens] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TUW OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tuw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tuw OCA], [https://pdbe.org/1tuw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tuw RCSB], [https://www.ebi.ac.uk/pdbsum/1tuw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tuw ProSAT]</span></td></tr>
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Structural and functional analysis of tetracenomycin F2 cyclase from Streptomyces glaucescens. A type II polyketide cyclase., Thompson TB, Katayama K, Watanabe K, Hutchinson CR, Rayment I, J Biol Chem. 2004 Sep 3;279(36):37956-63. Epub 2004 Jun 30. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15231835 15231835]
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</table>
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[[Category: Single protein]]
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== Function ==
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[https://www.uniprot.org/uniprot/TCMI_STRGA TCMI_STRGA] Required for conversion of tetracenomycin F2 to tetracenomycin D3.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tu/1tuw_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tuw ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Streptomyces glaucescens]]
[[Category: Streptomyces glaucescens]]
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[[Category: Hutchinson, C R.]]
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[[Category: Hutchinson CR]]
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[[Category: Katayama, K.]]
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[[Category: Katayama K]]
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[[Category: Rayment, I.]]
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[[Category: Rayment I]]
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[[Category: Thompson, T B.]]
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[[Category: Thompson TB]]
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[[Category: Watanabe, K.]]
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[[Category: Watanabe K]]
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[[Category: SO4]]
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[[Category: dimeric ??? ferredoxin-like fold tetracenomycin c biosynthesis]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:17:38 2008''
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Current revision

Structural and Functional Analysis of Tetracenomycin F2 Cyclase from Streptomyces glaucescens: A Type-II Polyketide Cyclase

PDB ID 1tuw

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