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1txj

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[[Image:1txj.jpg|left|200px]]<br /><applet load="1txj" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1txj, resolution 2.00&Aring;" />
 
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'''Crystal structure of translationally controlled tumour-associated protein (TCTP) from Plasmodium knowlesi'''<br />
 
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==Overview==
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==Crystal structure of translationally controlled tumour-associated protein (TCTP) from Plasmodium knowlesi==
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Parasites from the protozoan phylum Apicomplexa are responsible for diseases, such as malaria, toxoplasmosis and cryptosporidiosis, all of which have significantly higher rates of mortality and morbidity in economically underdeveloped regions of the world. Advances in vaccine development and drug discovery are urgently needed to control these diseases and can be facilitated by production of purified recombinant proteins from Apicomplexan genomes and determination of their 3D structures. To date, both heterologous expression and crystallization of Apicomplexan proteins have seen only limited success. In an effort to explore the effectiveness of producing and crystallizing proteins on a genome-scale using a standardized methodology, over 400 distinct Plasmodium falciparum target genes were chosen representing different cellular classes, along with select orthologues from four other Plasmodium species as well as Cryptosporidium parvum and Toxoplasma gondii. From a total of 1008 genes from the seven genomes, 304 (30.2%) produced purified soluble proteins and 97 (9.6%) crystallized, culminating in 36 crystal structures. These results demonstrate that, contrary to previous findings, a standardized platform using Escherichia coli can be effective for genome-scale production and crystallography of Apicomplexan proteins. Predictably, orthologous proteins from different Apicomplexan genomes behaved differently in expression, purification and crystallization, although the overall success rates of Plasmodium orthologues do not differ significantly. Their differences were effectively exploited to elevate the overall productivity to levels comparable to the most successful ongoing structural genomics projects: 229 of the 468 target genes produced purified soluble protein from one or more organisms, with 80 and 32 of the purified targets, respectively, leading to crystals and ultimately structures from one or more orthologues.
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<StructureSection load='1txj' size='340' side='right'caption='[[1txj]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1txj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_knowlesi Plasmodium knowlesi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TXJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TXJ FirstGlance]. <br>
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1TXJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Plasmodium_knowlesi Plasmodium knowlesi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TXJ OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1txj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1txj OCA], [https://pdbe.org/1txj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1txj RCSB], [https://www.ebi.ac.uk/pdbsum/1txj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1txj ProSAT]</span></td></tr>
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==Reference==
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</table>
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Genome-scale protein expression and structural biology of Plasmodium falciparum and related Apicomplexan organisms., Vedadi M, Lew J, Artz J, Amani M, Zhao Y, Dong A, Wasney GA, Gao M, Hills T, Brokx S, Qiu W, Sharma S, Diassiti A, Alam Z, Melone M, Mulichak A, Wernimont A, Bray J, Loppnau P, Plotnikova O, Newberry K, Sundararajan E, Houston S, Walker J, Tempel W, Bochkarev A, Kozieradzki I, Edwards A, Arrowsmith C, Roos D, Kain K, Hui R, Mol Biochem Parasitol. 2007 Jan;151(1):100-10. Epub 2006 Nov 13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17125854 17125854]
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== Function ==
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[https://www.uniprot.org/uniprot/TCTP_PLAKN TCTP_PLAKN] Involved in calcium binding and microtubule stabilization (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tx/1txj_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1txj ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Plasmodium knowlesi]]
[[Category: Plasmodium knowlesi]]
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[[Category: Single protein]]
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[[Category: Amani M]]
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[[Category: Amani, M.]]
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[[Category: Arrowsmith C]]
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[[Category: Arrowsmith, C.]]
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[[Category: Bray J]]
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[[Category: Bray, J.]]
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[[Category: Edwards A]]
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[[Category: Edwards, A.]]
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[[Category: Houston S]]
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[[Category: Houston, S.]]
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[[Category: Hui R]]
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[[Category: Hui, R.]]
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[[Category: Lew J]]
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[[Category: Lew, J.]]
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[[Category: Sharma S]]
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[[Category: SGC, Structural Genomics Consortium.]]
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[[Category: Skarina T]]
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[[Category: Sharma, S.]]
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[[Category: Sundstrom M]]
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[[Category: Skarina, T.]]
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[[Category: Vedadi M]]
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[[Category: Sundstrom, M.]]
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[[Category: Walker JR]]
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[[Category: Vedadi, M.]]
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[[Category: Wasney G]]
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[[Category: Walker, J R.]]
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[[Category: Wasney, G.]]
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[[Category: beta-sandwich]]
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[[Category: guanine nucleotide exchange factor]]
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[[Category: guanine nucleotide-free chaperones]]
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[[Category: helix-turn-helix]]
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[[Category: sgc]]
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[[Category: structural genomics consortium]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:18:29 2008''
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Current revision

Crystal structure of translationally controlled tumour-associated protein (TCTP) from Plasmodium knowlesi

PDB ID 1txj

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