1u3e

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(New page: 200px<br /><applet load="1u3e" size="450" color="white" frame="true" align="right" spinBox="true" caption="1u3e, resolution 2.92&Aring;" /> '''DNA binding and clea...)
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[[Image:1u3e.gif|left|200px]]<br /><applet load="1u3e" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1u3e, resolution 2.92&Aring;" />
 
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'''DNA binding and cleavage by the HNH homing endonuclease I-HmuI'''<br />
 
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==Overview==
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==DNA binding and cleavage by the HNH homing endonuclease I-HmuI==
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The structure of I-HmuI, which represents the last family of homing, endonucleases without a defining crystallographic structure, has been, determined in complex with its DNA target. A series of diverse protein, structural domains and motifs, contacting sequential stretches of, nucleotide bases, are distributed along the DNA target. I-HmuI contains an, N-terminal domain with a DNA-binding surface found in the I-PpoI homing, endonuclease and an associated HNH/N active site found in the bacterial, colicins, and a C-terminal DNA-binding domain previously observed in the, I-TevI homing endonuclease. The combination and exchange of these features, between protein families indicates that the genetic mobility associated, with homing endonucleases extends to the level of independent structural, domains. I-HmuI provides an unambiguous structural connection between the, His-Cys box endonucleases and the bacterial colicins, supporting the, hypothesis that these enzymes diverged from a common ancestral nuclease.
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<StructureSection load='1u3e' size='340' side='right'caption='[[1u3e]], [[Resolution|resolution]] 2.92&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1u3e]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_virus_SPO1 Bacillus virus SPO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U3E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U3E FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.92&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SR:STRONTIUM+ION'>SR</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u3e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u3e OCA], [https://pdbe.org/1u3e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u3e RCSB], [https://www.ebi.ac.uk/pdbsum/1u3e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u3e ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HMUI_BPSP1 HMUI_BPSP1]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u3/1u3e_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1u3e ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1U3E is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_sp6 Enterobacteria phage sp6] with MN, SR, EDO and TRS as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1U3E OCA].
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*[[Endonuclease 3D structures|Endonuclease 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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DNA binding and cleavage by the HNH homing endonuclease I-HmuI., Shen BW, Landthaler M, Shub DA, Stoddard BL, J Mol Biol. 2004 Sep 3;342(1):43-56. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15313606 15313606]
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[[Category: Bacillus virus SPO1]]
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[[Category: Enterobacteria phage sp6]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Landthaler M]]
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[[Category: Landthaler, M.]]
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[[Category: Shen BW]]
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[[Category: Shen, B.W.]]
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[[Category: Shub DA]]
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[[Category: Shub, D.A.]]
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[[Category: Stoddard BL]]
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[[Category: Stoddard, B.L.]]
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[[Category: EDO]]
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[[Category: MN]]
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[[Category: SR]]
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[[Category: TRS]]
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[[Category: helix-turn-helix dna binding domain]]
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[[Category: hnh catalytic motif]]
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[[Category: protein-dna complex]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 03:49:18 2007''
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Current revision

DNA binding and cleavage by the HNH homing endonuclease I-HmuI

PDB ID 1u3e

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