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1unk

From Proteopedia

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[[Image:1unk.gif|left|200px]]<br /><applet load="1unk" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1unk, resolution 1.8&Aring;" />
 
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'''STRUCTURE OF COLICIN E7 IMMUNITY PROTEIN'''<br />
 
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==Overview==
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==STRUCTURE OF COLICIN E7 IMMUNITY PROTEIN==
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Site-specific cleavage of mRNA has been identified in vivo for the polycistronic colicin E7 operon (ColE7), which occurs between G and A nucleotides located at the Asp52 codon (GAT) of the immunity gene (ceiE7). In vitro, this specific cleavage occurs only in the presence of the ceiE7 gene product (ImmE7). The crystal structure of dimeric ImmE7 has been determined at 1.8 A resolution by X-ray crystallographic analysis. We found that several residues located at the interface of dimeric ImmE7 bear surprising resemblance to the active sites of some RNases. These results suggest that dimeric ImmE7 may possess a novel RNase activity that cleaves its own mRNA at a specific site and thus autoregulates translational expression of the downstream celE7 gene as well as degradation of the upstream ceaE7 mRNA.
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<StructureSection load='1unk' size='340' side='right'caption='[[1unk]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1unk]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UNK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UNK FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1unk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1unk OCA], [https://pdbe.org/1unk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1unk RCSB], [https://www.ebi.ac.uk/pdbsum/1unk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1unk ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/IMM7_ECOLX IMM7_ECOLX] This protein is able to protect a cell, which harbors the plasmid ColE7 encoding colicin E7, against colicin E7, it binds specifically to the DNase-type colicin and inhibits its bactericidal activity. Dimeric ImmE7 may possess a RNase activity that cleaves its own mRNA at a specific site and thus autoregulates translational expression of the downstream ceiE7 gene as well as degradation of the upstream ceaE7 mRNA.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/un/1unk_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1unk ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1UNK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UNK OCA].
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*[[Colicin 3D structures|Colicin 3D structures]]
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*[[Colicin immunity protein 3D structures|Colicin immunity protein 3D structures]]
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==Reference==
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__TOC__
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A novel role of ImmE7 in the autoregulatory expression of the ColE7 operon and identification of possible RNase active sites in the crystal structure of dimeric ImmE7., Hsieh SY, Ko TP, Tseng MY, Ku W, Chak KF, Yuan HS, EMBO J. 1997 Mar 17;16(6):1444-54. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9135159 9135159]
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Chak, K F.]]
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[[Category: Chak K-F]]
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[[Category: Hsieh, S Y.]]
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[[Category: Hsieh S-Y]]
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[[Category: Ko, T P.]]
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[[Category: Ko T-P]]
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[[Category: Ku, W Y.]]
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[[Category: Ku W-Y]]
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[[Category: Tseng, M Y.]]
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[[Category: Tseng M-Y]]
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[[Category: Yuan, H S.]]
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[[Category: Yuan HS]]
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[[Category: dimeric structure]]
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[[Category: immunity protein]]
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[[Category: rnase active site]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:26:26 2008''
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Current revision

STRUCTURE OF COLICIN E7 IMMUNITY PROTEIN

PDB ID 1unk

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