1vhh

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(New page: 200px<br /><applet load="1vhh" size="450" color="white" frame="true" align="right" spinBox="true" caption="1vhh, resolution 1.7&Aring;" /> '''A POTENTIAL CATALYTIC...)
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[[Image:1vhh.gif|left|200px]]<br /><applet load="1vhh" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1vhh, resolution 1.7&Aring;" />
 
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'''A POTENTIAL CATALYTIC SITE WITHIN THE AMINO-TERMINAL SIGNALLING DOMAIN OF SONIC HEDGEHOG'''<br />
 
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==Overview==
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==A POTENTIAL CATALYTIC SITE WITHIN THE AMINO-TERMINAL SIGNALLING DOMAIN OF SONIC HEDGEHOG==
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Within the past few years, members of the hedgehog (hh) family of secreted, signalling proteins have emerged as the primary signals generated by, certain embryonic patterning centres. In vertebrate embryos, for example, sonic hedgehog expression in the notochord appears to be responsible for, the local and long-range induction of ventral cell types within the neural, tube and somites (reviewed in refs 1, 2). Protein products encoded by hh, family members are synthesized as precursors that undergo autoprocessing, to generate an amino-terminal domain that appears to be responsible for, both local and long-range signalling activities, and a carboxy-terminal, domain that contains the autoprocessing activity. As part of an effort to, understand how hh family members participate in cell-to-cell signalling, we have determined and report here the crystal structure at 1.7 A of the, amino-terminal domain of murine Sonic hedgehog (Shh-N). The structure, revealed a tetrahedrally coordinated zinc ion that appears to be, structurally analogous to the zinc coordination sites of zinc hydrolases, such as thermolysin and carboxypeptidase A. This previously unsuspected, catalytic site represents a distinct activity from the autoprocessing, activity that resides in the carboxy-terminal domain.
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<StructureSection load='1vhh' size='340' side='right'caption='[[1vhh]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1vhh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VHH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1VHH FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1vhh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vhh OCA], [https://pdbe.org/1vhh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1vhh RCSB], [https://www.ebi.ac.uk/pdbsum/1vhh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1vhh ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SHH_MOUSE SHH_MOUSE] Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the gli oncogene. Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction (By similarity).<ref>PMID:7736596</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vh/1vhh_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1vhh ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1VHH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with ZN and SO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1VHH OCA].
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*[[Sonic Hedgehog|Sonic Hedgehog]]
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*[[Sonic hedgehog 3D structures|Sonic hedgehog 3D structures]]
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==Reference==
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== References ==
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A potential catalytic site revealed by the 1.7-A crystal structure of the amino-terminal signalling domain of Sonic hedgehog., Hall TM, Porter JA, Beachy PA, Leahy DJ, Nature. 1995 Nov 9;378(6553):212-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7477329 7477329]
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Single protein]]
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[[Category: Beachy PA]]
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[[Category: Beachy, P.A.]]
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[[Category: Hall TMT]]
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[[Category: Hall, T.M.T.]]
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[[Category: Leahy DJ]]
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[[Category: Leahy, D.J.]]
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[[Category: Porter JA]]
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[[Category: Porter, J.A.]]
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[[Category: SO4]]
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[[Category: ZN]]
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[[Category: signalling protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 04:51:16 2007''
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Current revision

A POTENTIAL CATALYTIC SITE WITHIN THE AMINO-TERMINAL SIGNALLING DOMAIN OF SONIC HEDGEHOG

PDB ID 1vhh

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