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1vhr

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HUMAN VH1-RELATED DUAL-SPECIFICITY PHOSPHATASE

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OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1vhr"

@@ Line 1: / Line 1: @@
-[[Image:1vhr.gif|left|200px]]
- {{Structure
+==HUMAN VH1-RELATED DUAL-SPECIFICITY PHOSPHATASE==
-|PDB= 1vhr |SIZE=350|CAPTION= <scene name='initialview01'>1vhr</scene>, resolution 2.1&Aring;
+<StructureSection load='1vhr' size='340' side='right'caption='[[1vhr]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
-|SITE= <scene name='pdbsite=GAA:The+Loop+Containing+The+Putative+General+Acid+ASP+92'>GAA</scene>, <scene name='pdbsite=GAB:The+Loop+Containing+The+Putative+General+Acid+ASP+92'>GAB</scene>, <scene name='pdbsite=PLA:Phosphate+Binding+Loop+Including+Catalytic+Nucleophile+C+...'>PLA</scene>, <scene name='pdbsite=PLB:Phosphate+Binding+Loop+Including+Catalytic+Nucleophile+C+...'>PLB</scene>, <scene name='pdbsite=RCA:Designated+Recognition+Region+In+Primary+Reference.+Prop+...'>RCA</scene>, <scene name='pdbsite=RCB:Designated+Recognition+Region+In+Primary+Reference.+Prop+...'>RCB</scene>, <scene name='pdbsite=VRA:The+Region+Containing+High+Variation+Among+The+Dual-Spec+...'>VRA</scene> and <scene name='pdbsite=VRB:The+Region+Containing+High+Variation+Among+The+Dual-Spec+...'>VRB</scene>
+== Structural highlights ==
-|LIGAND= <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
+<table><tr><td colspan='2'>[[1vhr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VHR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1VHR FirstGlance]. <br>
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] </span>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
-|GENE=
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-|DOMAIN=
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1vhr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vhr OCA], [https://pdbe.org/1vhr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1vhr RCSB], [https://www.ebi.ac.uk/pdbsum/1vhr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1vhr ProSAT]</span></td></tr>
-|RELATEDENTRY=
+</table>
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vhr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vhr OCA], [http://www.ebi.ac.uk/pdbsum/1vhr PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1vhr RCSB]</span>
+== Function ==
-}}
+[https://www.uniprot.org/uniprot/DUS3_HUMAN DUS3_HUMAN] Shows activity both for tyrosine-protein phosphate and serine-protein phosphate, but displays a strong preference toward phosphotyrosines. Specifically dephosphorylates and inactivates ERK1 and ERK2.<ref>PMID:10224087</ref> <ref>PMID:11863439</ref>
+== Evolutionary Conservation ==
-'''HUMAN VH1-RELATED DUAL-SPECIFICITY PHOSPHATASE'''
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
-==Overview==
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vh/1vhr_consurf.spt"</scriptWhenChecked>
-Dual specificity protein phosphatases (DSPs) regulate mitogenic signal transduction and control the cell cycle. Here, the crystal structure of a human DSP, vaccinia H1-related phosphatase (or VHR), was determined at 2.1 angstrom resolution. A shallow active site pocket in VHR allows for the hydrolysis of phosphorylated serine, threonine, or tyrosine protein residues, whereas the deeper active site of protein tyrosine phosphatases (PTPs) restricts substrate specificity to only phosphotyrosine. Positively charged crevices near the active site may explain the enzyme's preference for substrates with two phosphorylated residues. The VHR structure defines a conserved structural scaffold for both DSPs and PTPs. A "recognition region," connecting helix alpha1 to strand beta1, may determine differences in substrate specificity between VHR, the PTPs, and other DSPs.
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
-==About this Structure==
+  </jmolCheckbox>
-VHR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VHR OCA].
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1vhr ConSurf].
+<div style="clear:both"></div>
-==Reference==
+== References ==
-Crystal structure of the dual specificity protein phosphatase VHR., Yuvaniyama J, Denu JM, Dixon JE, Saper MA, Science. 1996 May 31;272(5266):1328-31. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8650541 8650541]
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Protein-tyrosine-phosphatase]]
+[[Category: Large Structures]]
-[[Category: Single protein]]
+[[Category: Denu JM]]
-[[Category: Denu, J M.]]
+[[Category: Dixon JE]]
-[[Category: Dixon, J E.]]
+[[Category: Saper MA]]
-[[Category: Saper, M A.]]
+[[Category: Yuvaniyama J]]
-[[Category: Yuvaniyama, J.]]
-[[Category: hydrolase]]
-[[Category: protein dual-specificity phosphatase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:24:37 2008''

1vhr

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HUMAN VH1-RELATED DUAL-SPECIFICITY PHOSPHATASE

Structural highlights

Function

Evolutionary Conservation

References

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