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1vie

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[[Image:1vie.gif|left|200px]]<br /><applet load="1vie" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1vie, resolution 1.7&Aring;" />
 
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'''STRUCTURE OF DIHYDROFOLATE REDUCTASE'''<br />
 
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==Overview==
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==STRUCTURE OF DIHYDROFOLATE REDUCTASE==
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Bacteria expressing R67-plasmid encoded dihydrofolate reductase (R67 DHFR) exhibit high-level resistance to the antibiotic trimethoprim. Native R67 DHFR is a 34,000 M(r) homotetramer which exists in equilibrium with an inactive dimeric form. The structure of native R67 DHFR has now been solved at 1.7 A resolution and is unrelated to that of chromosomal DHFR. Homotetrameric R67 DHFR has an unusual pore, 25 A in length, passing through the middle of the molecule. Two folate molecules bind asymmetrically within the pore indicating that the enzyme's active site consists of symmetry related binding surfaces from all four identical units.
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<StructureSection load='1vie' size='340' side='right'caption='[[1vie]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1vie]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VIE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1VIE FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1vie FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vie OCA], [https://pdbe.org/1vie PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1vie RCSB], [https://www.ebi.ac.uk/pdbsum/1vie PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1vie ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DYR21_ECOLX DYR21_ECOLX] Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.
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==About this Structure==
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==See Also==
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1VIE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Active as [http://en.wikipedia.org/wiki/Dihydrofolate_reductase Dihydrofolate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.3 1.5.1.3] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VIE OCA].
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*[[Dihydrofolate reductase 3D structures|Dihydrofolate reductase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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A plasmid-encoded dihydrofolate reductase from trimethoprim-resistant bacteria has a novel D2-symmetric active site., Narayana N, Matthews DA, Howell EE, Nguyen-huu X, Nat Struct Biol. 1995 Nov;2(11):1018-25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7583655 7583655]
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[[Category: Dihydrofolate reductase]]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Howell, E E.]]
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[[Category: Howell EE]]
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[[Category: Matthews, D A.]]
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[[Category: Matthews DA]]
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[[Category: Narayana, N.]]
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[[Category: Narayana N]]
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[[Category: Xuong, N H.]]
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[[Category: Xuong N-H]]
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[[Category: methotrexate resistance]]
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[[Category: nadp]]
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[[Category: one-carbon metabolism]]
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[[Category: oxidoreductase]]
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[[Category: plasmid]]
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[[Category: trimethoprim resistance]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:35:39 2008''
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Current revision

STRUCTURE OF DIHYDROFOLATE REDUCTASE

PDB ID 1vie

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