1vpt

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[[Image:1vpt.jpg|left|200px]]
 
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{{Structure
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==AS11 VARIANT OF VACCINIA VIRUS PROTEIN VP39 IN COMPLEX WITH S-ADENOSYL-L-METHIONINE==
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|PDB= 1vpt |SIZE=350|CAPTION= <scene name='initialview01'>1vpt</scene>, resolution 1.8&Aring;
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<StructureSection load='1vpt' size='340' side='right'caption='[[1vpt]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene>
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<table><tr><td colspan='2'>[[1vpt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vaccinia_virus_WR Vaccinia virus WR]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VPT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1VPT FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Polynucleotide_adenylyltransferase Polynucleotide adenylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.19 2.7.7.19]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1vpt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vpt OCA], [https://pdbe.org/1vpt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1vpt RCSB], [https://www.ebi.ac.uk/pdbsum/1vpt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1vpt ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MCE_VACCW MCE_VACCW] Displays methyltransferase, positive regulation of the poly(A) polymerase and transcription elongation activities. Involved in the modification of both mRNA ends and in intermediate and late gene positive transcription elongation. At the mRNAs 5' end, methylates the ribose 2' OH group of the first transcribed nucleotide, thereby producing a 2'-O-methylpurine cap. At the 3' end, functions as a processivity factor which stimulates the activity of the viral poly(A) polymerase VP55 that creates mRNA's poly(A) tail. In the presence of VP39, VP55 does not dissociate from the RNA allowing tail elongation to around 250 adenylates.<ref>PMID:1313572</ref> <ref>PMID:1670500</ref> <ref>PMID:12359447</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vp/1vpt_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1vpt ConSurf].
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<div style="clear:both"></div>
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'''AS11 VARIANT OF VACCINIA VIRUS PROTEIN VP39 IN COMPLEX WITH S-ADENOSYL-L-METHIONINE'''
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==See Also==
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*[[Poly(A) polymerase 3D structures|Poly(A) polymerase 3D structures]]
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== References ==
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==Overview==
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<references/>
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VP39 is a bifunctional vaccinia virus protein that acts as both an mRNA cap-specific RNA 2'-O-methyltransferase and a poly(A) polymerase processivity factor. Here, we report the 1.85 A crystal structure of a VP39 variant complexed with its AdoMet cofactor. VP39 comprises a single core domain with structural similarity to the catalytic domains of other methyltransferases. Surface features and mutagenesis data suggest two possible RNA-binding sites with novel underlying architecture, one of which forms a cleft spanning the region adjacent to the methyltransferase active site. This report provides a prototypic structure for an RNA methyltransferase, a protein that interacts with the mRNA 5' cap, and an intact poxvirus protein.
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1VPT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Vaccinia_virus Vaccinia virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VPT OCA].
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[[Category: Vaccinia virus WR]]
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[[Category: Gershon PD]]
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==Reference==
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[[Category: Hodel AE]]
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The 1.85 A structure of vaccinia protein VP39: a bifunctional enzyme that participates in the modification of both mRNA ends., Hodel AE, Gershon PD, Shi X, Quiocho FA, Cell. 1996 Apr 19;85(2):247-56. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8612277 8612277]
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[[Category: Quiocho FA]]
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[[Category: Polynucleotide adenylyltransferase]]
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[[Category: Shi X]]
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[[Category: Single protein]]
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[[Category: Vaccinia virus]]
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[[Category: Gershon, P D.]]
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[[Category: Hodel, A E.]]
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[[Category: Quiocho, F A.]]
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[[Category: Shi, X.]]
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[[Category: SAM]]
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[[Category: methyltransferase]]
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[[Category: poly(a) polymerase]]
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[[Category: rna cap]]
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[[Category: vaccinia]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:48:07 2008''
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Current revision

AS11 VARIANT OF VACCINIA VIRUS PROTEIN VP39 IN COMPLEX WITH S-ADENOSYL-L-METHIONINE

PDB ID 1vpt

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