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1vqb

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[[Image:1vqb.jpg|left|200px]]
 
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{{Structure
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==GENE V PROTEIN (SINGLE-STRANDED DNA BINDING PROTEIN)==
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|PDB= 1vqb |SIZE=350|CAPTION= <scene name='initialview01'>1vqb</scene>, resolution 1.8&Aring;
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<StructureSection load='1vqb' size='340' side='right'caption='[[1vqb]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1vqb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterobacteria_phage_f1 Enterobacteria phage f1]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1bgh 1bgh]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VQB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1VQB FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1vqb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vqb OCA], [https://pdbe.org/1vqb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1vqb RCSB], [https://www.ebi.ac.uk/pdbsum/1vqb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1vqb ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vqb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vqb OCA], [http://www.ebi.ac.uk/pdbsum/1vqb PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1vqb RCSB]</span>
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[https://www.uniprot.org/uniprot/G5P_BPF1 G5P_BPF1] Binds to DNA in a highly cooperative manner without pronounced sequence specificity. During synthesis of the single-stranded (progeny) viral DNA, prevents the conversion into the double-stranded replicative form. G5P is displaced by the capsid protein G8P during phage assembly on the inner bacterial membrane (By similarity).
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}}
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vq/1vqb_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1vqb ConSurf].
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<div style="clear:both"></div>
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'''GENE V PROTEIN (SINGLE-STRANDED DNA BINDING PROTEIN)'''
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==See Also==
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*[[Single-stranded DNA-binding protein 3D structures|Single-stranded DNA-binding protein 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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The crystal structure of the dimeric gene V protein of bacteriophage f1 was determined using multiwavelength anomalous diffraction on the selenomethionine-containing wild-type and isoleucine-47--&gt;methionine mutant proteins with x-ray diffraction data phased to 2.5 A resolution. The structure of the wild-type protein has been refined to an R factor of 19.2% using native data to 1.8 A resolution. The structure of the gene V protein was used to obtain a model for the protein portion of the gene V protein-single-stranded DNA complex.
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==About this Structure==
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1VQB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_f1 Enterobacteria phage f1]. This structure supersedes the now removed PDB entry 1BGH. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VQB OCA].
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==Reference==
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Structure of the gene V protein of bacteriophage f1 determined by multiwavelength x-ray diffraction on the selenomethionyl protein., Skinner MM, Zhang H, Leschnitzer DH, Guan Y, Bellamy H, Sweet RM, Gray CW, Konings RN, Wang AH, Terwilliger TC, Proc Natl Acad Sci U S A. 1994 Mar 15;91(6):2071-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8134350 8134350]
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[[Category: Enterobacteria phage f1]]
[[Category: Enterobacteria phage f1]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Bellamy, H.]]
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[[Category: Bellamy H]]
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[[Category: Gray, C W.]]
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[[Category: Gray CW]]
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[[Category: Guan, Y.]]
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[[Category: Guan Y]]
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[[Category: Konings, R N.H.]]
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[[Category: Konings RNH]]
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[[Category: Leschnitzer, D H.]]
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[[Category: Leschnitzer DH]]
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[[Category: Skinner, M M.]]
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[[Category: Skinner MM]]
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[[Category: Sweet, R M.]]
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[[Category: Sweet RM]]
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[[Category: Terwilliger, T C.]]
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[[Category: Terwilliger TC]]
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[[Category: Wang, A H.J.]]
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[[Category: Wang AH-J]]
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[[Category: Zhang, H.]]
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[[Category: Zhang H]]
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[[Category: dna-binding protein]]
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[[Category: gene v]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:27:16 2008''
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Current revision

GENE V PROTEIN (SINGLE-STRANDED DNA BINDING PROTEIN)

PDB ID 1vqb

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