1wdc

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[[Image:1wdc.gif|left|200px]]<br /><applet load="1wdc" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1wdc, resolution 2.0&Aring;" />
 
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'''SCALLOP MYOSIN REGULATORY DOMAIN'''<br />
 
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==Overview==
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==SCALLOP MYOSIN REGULATORY DOMAIN==
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BACKGROUND: In contrast to the myosins of vertebrate skeletal muscle, molluscan myosins are regulated molecules whose enzymatic activity is switched on by the direct binding of Ca2+. The head portion (S1) of the molecule consists of a motor domain and a regulatory domain (RD) containing a 'regulatory' and an 'essential' light chain (RLC and ELC, respectively). The structures of scallop myosin RD with bound Ca2+, as well as the S1 fragment of chicken skeletal muscle myosin, have been determined previously to 2.8 A resolution. RESULTS: We have determined the structure at 2.0 A resolution of scallop myosin RD with bound Ca2+. The unusual coordination at the specific Ca(2+)-binding site in the ELC has now been clarified, as has the structural basis for Mg2+ binding to the RLC. A comparison of the scallop RD structure with that in the chicken S1 structure shows differences in the bending of the two RDs in two different places. CONCLUSIONS: Based on these structural results, a model for regulation is proposed in which the Ca(2+)-bound RD is a rigid structure, and transient flexibility of the Ca(2+)-free RD allows the myosin heads to make stabilizing intramolecular linkage which shut off the motor.
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<StructureSection load='1wdc' size='340' side='right'caption='[[1wdc]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1wdc]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Argopecten_irradians Argopecten irradians]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WDC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WDC FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wdc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wdc OCA], [https://pdbe.org/1wdc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wdc RCSB], [https://www.ebi.ac.uk/pdbsum/1wdc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wdc ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MYS_ARGIR MYS_ARGIR] Muscle contraction. Myosin is a protein that binds to F-actin and has ATPase activity that is activated by F-actin.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wd/1wdc_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wdc ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1WDC is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Argopecten_irradians Argopecten irradians] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WDC OCA].
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*[[Myosin 3D Structures|Myosin 3D Structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structure of the regulatory domain of scallop myosin at 2 A resolution: implications for regulation., Houdusse A, Cohen C, Structure. 1996 Jan 15;4(1):21-32. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8805510 8805510]
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[[Category: Argopecten irradians]]
[[Category: Argopecten irradians]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Cohen, C.]]
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[[Category: Cohen C]]
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[[Category: Houdusse, A.]]
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[[Category: Houdusse A]]
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[[Category: CA]]
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[[Category: MG]]
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[[Category: calcium binding protein]]
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[[Category: muscle protein]]
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[[Category: myosin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:43:03 2008''
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Current revision

SCALLOP MYOSIN REGULATORY DOMAIN

PDB ID 1wdc

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