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1x7h

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[[Image:1x7h.jpg|left|200px]]
 
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{{Structure
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==Actinorhodin Polyketide Ketoreductase, with NADPH bound==
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|PDB= 1x7h |SIZE=350|CAPTION= <scene name='initialview01'>1x7h</scene>, resolution 2.30&Aring;
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<StructureSection load='1x7h' size='340' side='right'caption='[[1x7h]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>
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<table><tr><td colspan='2'>[[1x7h]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X7H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1X7H FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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|GENE= actIII ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 Streptomyces coelicolor])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1x7h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x7h OCA], [https://pdbe.org/1x7h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1x7h RCSB], [https://www.ebi.ac.uk/pdbsum/1x7h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1x7h ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1x7g|1X7G]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1x7h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x7h OCA], [http://www.ebi.ac.uk/pdbsum/1x7h PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1x7h RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/ACT3_STRCO ACT3_STRCO]
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== Evolutionary Conservation ==
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'''Actinorhodin Polyketide Ketoreductase, with NADPH bound'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x7/1x7h_consurf.spt"</scriptWhenChecked>
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Aromatic polyketides are a class of natural products that include many pharmaceutically important aromatic compounds. Understanding the structure and function of PKS will provide clues to the molecular basis of polyketide biosynthesis specificity. Polyketide chain reduction by ketoreductase (KR) provides regio- and stereochemical diversity. Two cocrystal structures of actinorhodin polyketide ketoreductase (act KR) were solved to 2.3 A with either the cofactor NADP(+) or NADPH bound. The monomer fold is a highly conserved Rossmann fold. Subtle differences between structures of act KR and fatty acid KRs fine-tune the tetramer interface and substrate binding pocket. Comparisons of the NADP(+)- and NADPH-bound structures indicate that the alpha6-alpha7 loop region is highly flexible. The intricate proton-relay network in the active site leads to the proposed catalytic mechanism involving four waters, NADPH, and the active site tetrad Asn114-Ser144-Tyr157-Lys161. Acyl carrier protein and substrate docking models shed light on the molecular basis of KR regio- and stereoselectivity, as well as the differences between aromatic polyketide and fatty acid biosyntheses. Sequence comparison indicates that the above features are highly conserved among aromatic polyketide KRs. The structures of act KR provide an important step toward understanding aromatic PKS and will enhance our ability to design novel aromatic polyketide natural products with different reduction patterns.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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1X7H is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X7H OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1x7h ConSurf].
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<div style="clear:both"></div>
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==Reference==
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__TOC__
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Structural analysis of actinorhodin polyketide ketoreductase: cofactor binding and substrate specificity., Korman TP, Hill JA, Vu TN, Tsai SC, Biochemistry. 2004 Nov 23;43(46):14529-38. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15544323 15544323]
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</StructureSection>
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[[Category: Single protein]]
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[[Category: Large Structures]]
[[Category: Streptomyces coelicolor]]
[[Category: Streptomyces coelicolor]]
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[[Category: Hill, J A.]]
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[[Category: Hill JA]]
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[[Category: Korman, T P.]]
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[[Category: Korman TP]]
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[[Category: Vu, T N.]]
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[[Category: Vu TN]]
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[[Category: actinorhodin]]
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[[Category: combinatorial biosynthesis]]
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[[Category: ketoreductase]]
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[[Category: polyketide]]
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[[Category: short chain dehydrogenase/reductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:45:45 2008''
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Current revision

Actinorhodin Polyketide Ketoreductase, with NADPH bound

PDB ID 1x7h

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