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1x8s

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[[Image:1x8s.jpg|left|200px]]
 
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{{Structure
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==Structure of the Par-6 PDZ domain with a Pals1 internal ligand==
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|PDB= 1x8s |SIZE=350|CAPTION= <scene name='initialview01'>1x8s</scene>, resolution 2.503&Aring;
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<StructureSection load='1x8s' size='340' side='right'caption='[[1x8s]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1x8s]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X8S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1X8S FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.503&#8491;</td></tr>
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|GENE= PAR-6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 Drosophila melanogaster])
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1x8s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x8s OCA], [https://pdbe.org/1x8s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1x8s RCSB], [https://www.ebi.ac.uk/pdbsum/1x8s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1x8s ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1x8s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x8s OCA], [http://www.ebi.ac.uk/pdbsum/1x8s PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1x8s RCSB]</span>
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[https://www.uniprot.org/uniprot/O97111_DROME O97111_DROME]
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}}
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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'''Structure of the Par-6 PDZ domain with a Pals1 internal ligand'''
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x8/1x8s_consurf.spt"</scriptWhenChecked>
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==Overview==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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PDZ protein interaction domains are typically selective for C-terminal ligands, but non-C-terminal, 'internal' ligands have also been identified. The PDZ domain from the cell polarity protein Par-6 binds C-terminal ligands and an internal sequence from the protein Pals1/Stardust. The structure of the Pals1-Par-6 PDZ complex reveals that the PDZ ligand-binding site is deformed to allow for internal binding. Whereas binding of the Rho GTPase Cdc42 to a CRIB domain adjacent to the Par-6 PDZ regulates binding of C-terminal ligands, the conformational change that occurs upon binding of Pals1 renders its binding independent of Cdc42. These results suggest a mechanism by which the requirement for a C terminus can be readily bypassed by PDZ ligands and reveal a complex set of cooperative and competitive interactions in Par-6 that are likely to be important for cell polarity regulation.
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==About this Structure==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1x8s ConSurf].
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1X8S is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X8S OCA].
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<div style="clear:both"></div>
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__TOC__
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==Reference==
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</StructureSection>
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Internal recognition through PDZ domain plasticity in the Par-6-Pals1 complex., Penkert RR, DiVittorio HM, Prehoda KE, Nat Struct Mol Biol. 2004 Nov;11(11):1122-7. Epub 2004 Oct 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15475968 15475968]
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[[Category: Drosophila melanogaster]]
[[Category: Drosophila melanogaster]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: DiVittorio, H M.]]
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[[Category: DiVittorio HM]]
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[[Category: Penkert, R R.]]
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[[Category: Penkert RR]]
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[[Category: Prehoda, K E.]]
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[[Category: Prehoda KE]]
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[[Category: cell cycle]]
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[[Category: par-6]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:46:20 2008''
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Current revision

Structure of the Par-6 PDZ domain with a Pals1 internal ligand

PDB ID 1x8s

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