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1x8w

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[[Image:1x8w.gif|left|200px]]
 
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{{Structure
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==Structure of the Tetrahymena Ribozyme: Base Triple Sandwich and Metal Ion at the Active Site==
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|PDB= 1x8w |SIZE=350|CAPTION= <scene name='initialview01'>1x8w</scene>, resolution 3.8&Aring;
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<StructureSection load='1x8w' size='340' side='right'caption='[[1x8w]], [[Resolution|resolution]] 3.80&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene>
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<table><tr><td colspan='2'>[[1x8w]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Tetrahymena_thermophila Tetrahymena thermophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X8W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1X8W FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.8&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1x8w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x8w OCA], [https://pdbe.org/1x8w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1x8w RCSB], [https://www.ebi.ac.uk/pdbsum/1x8w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1x8w ProSAT]</span></td></tr>
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</table>
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'''Structure of the Tetrahymena Ribozyme: Base Triple Sandwich and Metal Ion at the Active Site'''
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==See Also==
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*[[Ribozyme 3D structures|Ribozyme 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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The Tetrahymena intron is an RNA catalyst, or ribozyme. As part of its self-splicing reaction, this ribozyme catalyzes phosphoryl transfer between guanosine and a substrate RNA strand. Here we report the refined crystal structure of an active Tetrahymena ribozyme in the absence of its RNA substrate at 3.8 A resolution. The 3'-terminal guanosine (omegaG), which serves as the attacking group for RNA cleavage, forms a coplanar base triple with the G264-C311 base pair, and this base triple is sandwiched by three other base triples. In addition, a metal ion is present in the active site, contacting or positioned close to the ribose of the omegaG and five phosphates. All of these phosphates have been shown to be important for catalysis. Therefore, we provide a picture of how the ribozyme active site positions both a catalytic metal ion and the nucleophilic guanosine for catalysis prior to binding its RNA substrate.
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[[Category: Large Structures]]
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[[Category: Tetrahymena thermophila]]
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==About this Structure==
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[[Category: Cech TR]]
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1X8W is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X8W OCA].
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[[Category: Gooding AR]]
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[[Category: Guo F]]
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==Reference==
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Structure of the Tetrahymena ribozyme: base triple sandwich and metal ion at the active site., Guo F, Gooding AR, Cech TR, Mol Cell. 2004 Nov 5;16(3):351-62. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15525509 15525509]
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[[Category: Protein complex]]
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[[Category: Cech, T R.]]
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[[Category: Gooding, A R.]]
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[[Category: Guo, F.]]
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[[Category: MG]]
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[[Category: active site]]
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[[Category: base triple]]
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[[Category: catalytic mechanism]]
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[[Category: catalytic rna]]
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[[Category: conformational change]]
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[[Category: crystal structure]]
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[[Category: group i intron]]
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[[Category: guanosine binding site]]
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[[Category: metal ion]]
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[[Category: ribozyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:06:33 2008''
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Current revision

Structure of the Tetrahymena Ribozyme: Base Triple Sandwich and Metal Ion at the Active Site

PDB ID 1x8w

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