This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1x9m

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:48, 14 February 2024) (edit) (undo)
 
(10 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:1x9m.png|left|200px]]
 
-
<!--
+
==T7 DNA polymerase in complex with an N-2-acetylaminofluorene-adducted DNA==
-
The line below this paragraph, containing "STRUCTURE_1x9m", creates the "Structure Box" on the page.
+
<StructureSection load='1x9m' size='340' side='right'caption='[[1x9m]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[1x9m]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Escherichia_phage_T7 Escherichia phage T7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X9M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1X9M FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2DT:3-DEOXYTHYMIDINE-5-MONOPHOSPHATE'>2DT</scene>, <scene name='pdbligand=8FG:N-(5-PHOSPHO-2-DEOXYGUANOSIN-8-YL)-2-ACETYLAMINOFLUORENE'>8FG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-
{{STRUCTURE_1x9m| PDB=1x9m | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1x9m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x9m OCA], [https://pdbe.org/1x9m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1x9m RCSB], [https://www.ebi.ac.uk/pdbsum/1x9m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1x9m ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/THIO_ECOLI THIO_ECOLI] Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x9/1x9m_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1x9m ConSurf].
 +
<div style="clear:both"></div>
-
===T7 DNA polymerase in complex with an N-2-acetylaminofluorene-adducted DNA===
+
==See Also==
-
 
+
*[[DNA polymerase 3D structures|DNA polymerase 3D structures]]
-
 
+
*[[Thioredoxin 3D structures|Thioredoxin 3D structures]]
-
<!--
+
__TOC__
-
The line below this paragraph, {{ABSTRACT_PUBMED_15528277}}, adds the Publication Abstract to the page
+
</StructureSection>
-
(as it appears on PubMed at http://www.pubmed.gov), where 15528277 is the PubMed ID number.
+
-
-->
+
-
{{ABSTRACT_PUBMED_15528277}}
+
-
 
+
-
==About this Structure==
+
-
1X9M is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t7 Enterobacteria phage t7] and [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X9M OCA].
+
-
 
+
-
==Reference==
+
-
Crystal structures of 2-acetylaminofluorene and 2-aminofluorene in complex with T7 DNA polymerase reveal mechanisms of mutagenesis., Dutta S, Li Y, Johnson D, Dzantiev L, Richardson CC, Romano LJ, Ellenberger T, Proc Natl Acad Sci U S A. 2004 Nov 16;101(46):16186-91. Epub 2004 Nov 4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15528277 15528277]
+
-
[[Category: DNA-directed DNA polymerase]]
+
-
[[Category: Enterobacteria phage t7]]
+
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
-
[[Category: Protein complex]]
+
[[Category: Escherichia phage T7]]
-
[[Category: Dutta, S.]]
+
[[Category: Large Structures]]
-
[[Category: Dzantiev, L.]]
+
[[Category: Dutta S]]
-
[[Category: Ellenberger, T.]]
+
[[Category: Dzantiev L]]
-
[[Category: Johnson, D.]]
+
[[Category: Ellenberger T]]
-
[[Category: Li, Y.]]
+
[[Category: Johnson D]]
-
[[Category: Richardson, C C.]]
+
[[Category: Li Y]]
-
[[Category: Romano, L J.]]
+
[[Category: Richardson CC]]
-
[[Category: Dna ploymerase]]
+
[[Category: Romano LJ]]
-
[[Category: Mutagenesis]]
+
-
[[Category: N-2-acetylaminofluorene]]
+
-
[[Category: Replication block]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 02:06:25 2008''
+

Current revision

T7 DNA polymerase in complex with an N-2-acetylaminofluorene-adducted DNA

PDB ID 1x9m

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1x9m"
Personal tools