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1xam

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[[Image:1xam.gif|left|200px]]
 
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{{Structure
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==Cobalt hexammine induced tautameric shift in Z-DNA: structure of d(CGCGCA).d(TGCGCG) in two crystal forms.==
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|PDB= 1xam |SIZE=350|CAPTION= <scene name='initialview01'>1xam</scene>, resolution 1.86&Aring;
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<StructureSection load='1xam' size='340' side='right'caption='[[1xam]], [[Resolution|resolution]] 1.86&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=NCO:COBALT HEXAMMINE ION'>NCO</scene>
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<table><tr><td colspan='2'>[[1xam]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XAM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XAM FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.86&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NCO:COBALT+HEXAMMINE(III)'>NCO</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xam FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xam OCA], [https://pdbe.org/1xam PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xam RCSB], [https://www.ebi.ac.uk/pdbsum/1xam PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xam ProSAT]</span></td></tr>
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</table>
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'''Cobalt hexammine induced tautameric shift in Z-DNA: structure of d(CGCGCA).d(TGCGCG) in two crystal forms.'''
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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==Overview==
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[[Category: Gautham N]]
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We report here the crystal structure of the DNA hexamer duplex d(CGCGCA)*d(TGCGCG) at 1.71 A resolution. The crystals, in orthorhombic space group, were grown in the presence of cobalt hexammine, a known inducer of the left-handed Z form of DNA. The interaction of this ion with the DNA helix results in a change of the adenine base from the common amino tautomeric form to the imino tautomer. Consequently the A:T base pair is disrupted from the normal Watson-Crick base pairing to a 'wobble' like base pairing. This change is accommodated easily within the helix, and the helical parameters are those expected for Z-DNA. When the cobalt hexammine concentration is decreased slightly in the crystallization conditions, the duplex crystallizes in a different, hexagonal space group, with two hexamer duplexes in the asymmetric unit. One of these is situated on a crystallographic 6-fold screw axis, leading to disorder. The tautomeric shift is not observed in this space group. We show that the change in inter-helix interactions that lead to the two different space groups probably arise from the small decrease in ion concentration, and consequently disordered positions for the ion.
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[[Category: Rajan SS]]
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[[Category: Thiyagarajan S]]
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==About this Structure==
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1XAM is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XAM OCA].
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==Reference==
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Cobalt hexammine induced tautomeric shift in Z-DNA: the structure of d(CGCGCA)*d(TGCGCG) in two crystal forms., Thiyagarajan S, Rajan SS, Gautham N, Nucleic Acids Res. 2004 Nov 8;32(19):5945-53. Print 2004. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15534365 15534365]
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[[Category: Protein complex]]
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[[Category: Gautham, N.]]
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[[Category: Rajan, S S.]]
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[[Category: Thiyagarajan, S.]]
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[[Category: NCO]]
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[[Category: double helix]]
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[[Category: z-dna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:07:17 2008''
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Current revision

Cobalt hexammine induced tautameric shift in Z-DNA: structure of d(CGCGCA).d(TGCGCG) in two crystal forms.

PDB ID 1xam

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