1xcc

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[[Image:1xcc.jpg|left|200px]]
 
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==1-Cys peroxidoxin from Plasmodium Yoelli==
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The line below this paragraph, containing "STRUCTURE_1xcc", creates the "Structure Box" on the page.
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<StructureSection load='1xcc' size='340' side='right'caption='[[1xcc]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1xcc]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_yoelii Plasmodium yoelii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XCC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XCC FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xcc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xcc OCA], [https://pdbe.org/1xcc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xcc RCSB], [https://www.ebi.ac.uk/pdbsum/1xcc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xcc ProSAT]</span></td></tr>
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{{STRUCTURE_1xcc| PDB=1xcc | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q86SB3_PLAYE Q86SB3_PLAYE] Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively.[PIRNR:PIRNR000239]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xc/1xcc_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xcc ConSurf].
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<div style="clear:both"></div>
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'''1-Cys peroxidoxin from Plasmodium Yoelli'''
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==See Also==
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*[[Peroxiredoxin 3D structures|Peroxiredoxin 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Parasites from the protozoan phylum Apicomplexa are responsible for diseases, such as malaria, toxoplasmosis and cryptosporidiosis, all of which have significantly higher rates of mortality and morbidity in economically underdeveloped regions of the world. Advances in vaccine development and drug discovery are urgently needed to control these diseases and can be facilitated by production of purified recombinant proteins from Apicomplexan genomes and determination of their 3D structures. To date, both heterologous expression and crystallization of Apicomplexan proteins have seen only limited success. In an effort to explore the effectiveness of producing and crystallizing proteins on a genome-scale using a standardized methodology, over 400 distinct Plasmodium falciparum target genes were chosen representing different cellular classes, along with select orthologues from four other Plasmodium species as well as Cryptosporidium parvum and Toxoplasma gondii. From a total of 1008 genes from the seven genomes, 304 (30.2%) produced purified soluble proteins and 97 (9.6%) crystallized, culminating in 36 crystal structures. These results demonstrate that, contrary to previous findings, a standardized platform using Escherichia coli can be effective for genome-scale production and crystallography of Apicomplexan proteins. Predictably, orthologous proteins from different Apicomplexan genomes behaved differently in expression, purification and crystallization, although the overall success rates of Plasmodium orthologues do not differ significantly. Their differences were effectively exploited to elevate the overall productivity to levels comparable to the most successful ongoing structural genomics projects: 229 of the 468 target genes produced purified soluble protein from one or more organisms, with 80 and 32 of the purified targets, respectively, leading to crystals and ultimately structures from one or more orthologues.
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[[Category: Large Structures]]
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==About this Structure==
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1XCC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Plasmodium_yoelii Plasmodium yoelii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XCC OCA].
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==Reference==
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Genome-scale protein expression and structural biology of Plasmodium falciparum and related Apicomplexan organisms., Vedadi M, Lew J, Artz J, Amani M, Zhao Y, Dong A, Wasney GA, Gao M, Hills T, Brokx S, Qiu W, Sharma S, Diassiti A, Alam Z, Melone M, Mulichak A, Wernimont A, Bray J, Loppnau P, Plotnikova O, Newberry K, Sundararajan E, Houston S, Walker J, Tempel W, Bochkarev A, Kozieradzki I, Edwards A, Arrowsmith C, Roos D, Kain K, Hui R, Mol Biochem Parasitol. 2007 Jan;151(1):100-10. Epub 2006 Nov 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17125854 17125854]
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[[Category: Plasmodium yoelii]]
[[Category: Plasmodium yoelii]]
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[[Category: Single protein]]
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[[Category: Amani M]]
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[[Category: Amani, M.]]
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[[Category: Arrowsmith C]]
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[[Category: Arrowsmith, C.]]
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[[Category: Bochkarev A]]
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[[Category: Bochkarev, A.]]
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[[Category: Bray J]]
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[[Category: Bray, J.]]
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[[Category: Edwards A]]
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[[Category: Edwards, A.]]
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[[Category: Houston S]]
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[[Category: Houston, S.]]
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[[Category: Hui R]]
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[[Category: Hui, R.]]
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[[Category: Lew J]]
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[[Category: Lew, J.]]
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[[Category: Sharma S]]
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[[Category: SGC, Structural Genomics Consortium.]]
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[[Category: Sundstrom M]]
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[[Category: Sharma, S.]]
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[[Category: Vedadi M]]
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[[Category: Sundstrom, M.]]
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[[Category: Wasney G]]
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[[Category: Vedadi, M.]]
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[[Category: Xu X]]
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[[Category: Wasney, G.]]
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[[Category: Xu, X.]]
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[[Category: Sgc]]
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[[Category: Structural genomic]]
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[[Category: Structural genomics consortium]]
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[[Category: Unknown function]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 14:50:49 2008''
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Current revision

1-Cys peroxidoxin from Plasmodium Yoelli

PDB ID 1xcc

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