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1xgs

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[[Image:1xgs.gif|left|200px]]<br />
 
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<applet load="1xgs" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1xgs, resolution 1.75&Aring;" />
 
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'''METHIONINE AMINOPEPTIDASE FROM HYPERTHERMOPHILE PYROCOCCUS FURIOSUS'''<br />
 
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==Overview==
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==METHIONINE AMINOPEPTIDASE FROM HYPERTHERMOPHILE PYROCOCCUS FURIOSUS==
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The structure of methionine aminopeptidase from hyperthermophile, Pyrococcus furiosus (PfMAP) with an optimal growth temperature of 100, degreesC was determined by the multiple isomorphous replacement method and, refined in three different crystal forms, one monoclinic and two, hexagonal, at resolutions of 2.8, 2.9, and 3.5 A. The resolution of the, monoclinic crystal form was extended to 1.75 A by water-mediated, transformation to a low-humidity form, and the obtained diffraction data, used for high-resolution structure refinement. This is the first, description of a eukaryotic type methionine aminopeptidase structure. The, PfMAP molecule is composed of two domains, a catalytic domain and an, insertion domain, connected via two antiparallel beta-strands. The, catalytic domain, which ... [[http://ispc.weizmann.ac.il/pmbin/getpm?9811545 (full description)]]
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<StructureSection load='1xgs' size='340' side='right'caption='[[1xgs]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1xgs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XGS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XGS FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xgs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xgs OCA], [https://pdbe.org/1xgs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xgs RCSB], [https://www.ebi.ac.uk/pdbsum/1xgs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xgs ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MAP2_PYRFU MAP2_PYRFU] Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).[HAMAP-Rule:MF_01975]<ref>PMID:9399590</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xg/1xgs_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xgs ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1XGS is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]] with CO as [[http://en.wikipedia.org/wiki/ligand ligand]]. Structure known Active Sites: ACA and ACB. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1XGS OCA]].
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*[[Aminopeptidase 3D structures|Aminopeptidase 3D structures]]
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== References ==
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==Reference==
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<references/>
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Crystal structure of methionine aminopeptidase from hyperthermophile, Pyrococcus furiosus., Tahirov TH, Oki H, Tsukihara T, Ogasahara K, Yutani K, Ogata K, Izu Y, Tsunasawa S, Kato I, J Mol Biol. 1998 Nov 20;284(1):101-24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9811545 9811545]
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Pyrococcus furiosus]]
[[Category: Pyrococcus furiosus]]
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[[Category: Single protein]]
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[[Category: Tahirov TH]]
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[[Category: Tahirov, T.H.]]
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[[Category: Tsukihara T]]
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[[Category: Tsukihara, T.]]
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[[Category: CO]]
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[[Category: aminopeptidase]]
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[[Category: hyperthermophile]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 10:57:33 2007''
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Current revision

METHIONINE AMINOPEPTIDASE FROM HYPERTHERMOPHILE PYROCOCCUS FURIOSUS

PDB ID 1xgs

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