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1xhz

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(New page: 200px<br /><applet load="1xhz" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xhz, resolution 2.70&Aring;" /> '''Phi29 DNA polymerase...)
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[[Image:1xhz.gif|left|200px]]<br /><applet load="1xhz" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1xhz, resolution 2.70&Aring;" />
 
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'''Phi29 DNA polymerase, orthorhombic crystal form, ssDNA complex'''<br />
 
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==Overview==
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==Phi29 DNA polymerase, orthorhombic crystal form, ssDNA complex==
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The DNA polymerase from phage phi29 is a B family polymerase that, initiates replication using a protein as a primer, attaching the first, nucleotide of the phage genome to the hydroxyl of a specific serine of the, priming protein. The crystal structure of phi29 DNA polymerase determined, at 2.2 A resolution provides explanations for its extraordinary, processivity and strand displacement activities. Homology modeling, suggests that downstream template DNA passes through a tunnel prior to, entering the polymerase active site. This tunnel is too small to, accommodate double-stranded DNA and requires the separation of template, and nontemplate strands. Members of the B family of DNA polymerases that, use protein primers contain two sequence insertions: one forms a domain, not previously observed in polymerases, while the second resembles the, specificity loop of T7 RNA polymerase. The high processivity of phi29 DNA, polymerase may be explained by its topological encirclement of both the, downstream template and the upstream duplex DNA.
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<StructureSection load='1xhz' size='340' side='right'caption='[[1xhz]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1xhz]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_virus_phi29 Bacillus virus phi29]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XHZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XHZ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xhz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xhz OCA], [https://pdbe.org/1xhz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xhz RCSB], [https://www.ebi.ac.uk/pdbsum/1xhz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xhz ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DPOL_BPPH2 DPOL_BPPH2] This polymerase possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'- to 5'-direction.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xh/1xhz_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xhz ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1XHZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Vibrio_phage_f237 Vibrio phage f237]. Active as [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1XHZ OCA].
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*[[DNA polymerase 3D structures|DNA polymerase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Insights into strand displacement and processivity from the crystal structure of the protein-primed DNA polymerase of bacteriophage phi29., Kamtekar S, Berman AJ, Wang J, Lazaro JM, de Vega M, Blanco L, Salas M, Steitz TA, Mol Cell. 2004 Nov 19;16(4):609-18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15546620 15546620]
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[[Category: Bacillus virus phi29]]
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[[Category: DNA-directed DNA polymerase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Berman AJ]]
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[[Category: Vibrio phage f237]]
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[[Category: Blanco L]]
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[[Category: Berman, A.J.]]
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[[Category: Kamtekar S]]
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[[Category: Blanco, L.]]
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[[Category: Lazaro JM]]
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[[Category: Kamtekar, S.]]
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[[Category: Salas M]]
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[[Category: Lazaro, J.M.]]
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[[Category: Steitz TA]]
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[[Category: Salas, M.]]
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[[Category: Wang J]]
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[[Category: Steitz, T.A.]]
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[[Category: De Vega M]]
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[[Category: Vega, M.de.]]
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[[Category: Wang, J.]]
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[[Category: dna polymerase]]
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[[Category: processivity]]
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[[Category: protein-primed]]
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[[Category: replication]]
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[[Category: strand displacement]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 06:05:00 2007''
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Current revision

Phi29 DNA polymerase, orthorhombic crystal form, ssDNA complex

PDB ID 1xhz

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