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1xo5

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[[Image:1xo5.jpg|left|200px]]
 
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{{Structure
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==Crystal structure of CIB1, an EF-hand, integrin and kinase-binding protein==
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|PDB= 1xo5 |SIZE=350|CAPTION= <scene name='initialview01'>1xo5</scene>, resolution 1.99&Aring;
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<StructureSection load='1xo5' size='340' side='right'caption='[[1xo5]], [[Resolution|resolution]] 1.99&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene>
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<table><tr><td colspan='2'>[[1xo5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XO5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XO5 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.99&#8491;</td></tr>
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|GENE= CIB1, PRKDCIP, KIP, CIB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xo5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xo5 OCA], [https://pdbe.org/1xo5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xo5 RCSB], [https://www.ebi.ac.uk/pdbsum/1xo5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xo5 ProSAT]</span></td></tr>
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</table>
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'''Crystal structure of CIB1, an EF-hand, integrin and kinase-binding protein'''
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== Function ==
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[https://www.uniprot.org/uniprot/CIB1_HUMAN CIB1_HUMAN] May convert the inactive conformation of integrin alpha-IIb/beta3 to an active form through binding to the integrin cytoplasmic domain. Induces cell migration and spreading mediated through integrin (possibly via focal adhesion complexes). Functions as a negative regulator of stress activated MAP kinase (MAPK) signaling pathways. May play a role in regulation of apoptosis. Interacts with and up-regulates PTK2/FAK1 activity. Down regulates inositol 1,4,5-trisphosphate receptor-dependent calcium signaling. Participates in endomitotic cell cycle, a form of mitosis in which both karyokinesis and cytokinesis are interrupted and is a hallmark of megakaryocyte differentiation.<ref>PMID:12714504</ref> <ref>PMID:12881299</ref> <ref>PMID:15685448</ref> <ref>PMID:18627437</ref> <ref>PMID:19805025</ref> <ref>PMID:21264284</ref>
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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CIB1 (CIB) is an EF-hand-containing protein that binds multiple effector proteins, including the platelet alphaIIbbeta3 integrin and several serine/threonine kinases and potentially modulates their function. The crystal structure for Ca(2+)-bound CIB1 has been determined at 2.0 A resolution and reveals a compact alpha-helical protein containing four EF-hands, the last two of which bind calcium ions in the standard fashion seen in many other EF-hand proteins. CIB1 shares high structural similarity with calcineurin B and the neuronal calcium sensor (NCS) family of EF-hand-containing proteins. Most importantly, like calcineurin B and NCS proteins, which possess a large hydrophobic pocket necessary for ligand binding, CIB1 contains a hydrophobic pocket that has been implicated in ligand binding by previous mutational analysis. However, unlike several NCS proteins, Ca(2+)-bound CIB1 is largely monomeric whether bound to a relevant peptide ligand or ligand-free. Differences in structure, oligomeric state, and phylogeny define a new family of CIB1-related proteins that extends from arthropods to humans.
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Check<jmol>
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<jmolCheckbox>
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==Disease==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xo/1xo5_consurf.spt"</scriptWhenChecked>
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Known disease associated with this structure: Multiple endocrine neoplasia, type IV OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=600778 600778]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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1XO5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XO5 OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xo5 ConSurf].
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<div style="clear:both"></div>
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==Reference==
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== References ==
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Structural and biochemical characterization of CIB1 delineates a new family of EF-hand-containing proteins., Gentry HR, Singer AU, Betts L, Yang C, Ferrara JD, Sondek J, Parise LV, J Biol Chem. 2005 Mar 4;280(9):8407-15. Epub 2004 Dec 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15574431 15574431]
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Betts, L.]]
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[[Category: Betts L]]
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[[Category: Ferrara, J D.]]
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[[Category: Ferrara JD]]
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[[Category: Gentry, H R.]]
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[[Category: Gentry HR]]
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[[Category: Parise, L V.]]
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[[Category: Parise LV]]
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[[Category: Singer, A U.]]
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[[Category: Singer AU]]
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[[Category: Sondek, J.]]
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[[Category: Sondek J]]
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[[Category: Yang, C.]]
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[[Category: Yang C]]
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[[Category: CA]]
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[[Category: calcium and integrin binding]]
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[[Category: calmyrin]]
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[[Category: ef-hand]]
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[[Category: kinase interacting protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:12:23 2008''
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Current revision

Crystal structure of CIB1, an EF-hand, integrin and kinase-binding protein

PDB ID 1xo5

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