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1y4t

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(New page: 200px<br /><applet load="1y4t" size="450" color="white" frame="true" align="right" spinBox="true" caption="1y4t, resolution 1.80&Aring;" /> '''Ferric binding prote...)
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[[Image:1y4t.gif|left|200px]]<br /><applet load="1y4t" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1y4t, resolution 1.80&Aring;" />
 
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'''Ferric binding protein from Campylobacter jejuni'''<br />
 
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==Overview==
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==Ferric binding protein from Campylobacter jejuni==
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Campylobacter jejuni, the leading cause of human gastroenteritis, expresses a ferric binding protein (cFbpA) that in many pathogenic, bacteria functions to acquire iron as part of their virulence repertoire., Recombinant cFbpA is isolated with ferric iron bound from Escherichia, coli. The crystal structure of cFbpA reveals unprecedented iron, coordination by only five protein ligands. The histidine and one tyrosine, are derived from the N-terminal domain, whereas the three remaining, tyrosine ligands are from the C-terminal domain. Surprisingly, a, synergistic anion present in all other characterized ferric transport, proteins is not observed in the cFbpA iron-binding site, suggesting a, novel role for this protein in iron uptake. Furthermore, cFbpA is shown to, bind iron with high affinity similar to Neisserial FbpA and exhibits an, unusual preference for ferrous iron (oxidized subsequently to the ferric, form) or ferric iron chelated by oxalate. Sequence and structure analyses, reveal that cFbpA is a member of a new class of ferric binding proteins, that includes homologs from invasive and intracellular bacteria as well as, cyanobacteria. Overall, six classes are defined based on clustering within, the tree and by their putative iron coordination. The absence of a, synergistic anion in the iron coordination sphere of cFbpA also suggests, an alternative model of evolution for FbpA homologs involving an early, iron-binding ancestor instead of a requirement for a preexisting, anion-binding ancestor.
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<StructureSection load='1y4t' size='340' side='right'caption='[[1y4t]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1y4t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Campylobacter_jejuni Campylobacter jejuni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y4T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Y4T FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1y4t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y4t OCA], [https://pdbe.org/1y4t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1y4t RCSB], [https://www.ebi.ac.uk/pdbsum/1y4t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1y4t ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q0PBW4_CAMJE Q0PBW4_CAMJE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/y4/1y4t_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1y4t ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1Y4T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Campylobacter_jejuni Campylobacter jejuni] with FE as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1Y4T OCA].
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*[[Ferric-binding protein|Ferric-binding protein]]
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__TOC__
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==Reference==
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</StructureSection>
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Anion-independent iron coordination by the Campylobacter jejuni ferric binding protein., Tom-Yew SA, Cui DT, Bekker EG, Murphy ME, J Biol Chem. 2005 Mar 11;280(10):9283-90. Epub 2004 Dec 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15613474 15613474]
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[[Category: Campylobacter jejuni]]
[[Category: Campylobacter jejuni]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Bekker, E.G.]]
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[[Category: Bekker EG]]
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[[Category: Cui, D.T.]]
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[[Category: Cui DT]]
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[[Category: Murphy, M.E.P.]]
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[[Category: Murphy MEP]]
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[[Category: Tom-Yew, S.A.L.]]
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[[Category: Tom-Yew SAL]]
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[[Category: FE]]
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[[Category: iron-tyrosinate]]
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[[Category: periplasmic binding protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 03:29:16 2007''
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Current revision

Ferric binding protein from Campylobacter jejuni

PDB ID 1y4t

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