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1yad

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Structure of TenI from Bacillus subtilis

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Retrieved from "http://52.214.119.220/wiki/index.php/1yad"

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-[[Image:1yad.gif|left|200px]]<br /><applet load="1yad" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1yad, resolution 2.10&Aring;" />
-'''Structure of TenI from Bacillus subtilis'''<br />
-==Overview==
+==Structure of TenI from Bacillus subtilis==
-Bacillus subtilis gene products TenA and TenI have been implicated in, regulating the production of extracellular proteases, but their role in, the regulation process remains unclear. The structural characterization of, these proteins was undertaken to help provide insight into their function., We have determined the structure of TenA alone and in complex with, 4-amino-2-methyl-5-hydroxymethylpyrimidine, and we demonstrate that TenA, is a thiaminase II. The TenA structure suggests that the degradation of, thiamin by TenA likely proceeds via the same addition-elimination, mechanism described for thiaminase I. Three active-site residues, Asp44, Cys135, and Glu205, are likely involved in substrate binding and catalysis, based on the enzyme/product complex structure and the conservation of, these residues within TenA sequences. We have also determined the, structure of TenI. Although TenI shows significant structural homology to, thiamin phosphate synthase, it has no known enzymatic function. The, structure suggests that TenI is unable to bind thiamin phosphate, largely, resulting from the presence of leucine at position 119, while the, corresponding residue in thiamin phosphate synthase is glycine.
+<StructureSection load='1yad' size='340' side='right'caption='[[1yad]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
+== Structural highlights ==
-==About this Structure==
+<table><tr><td colspan='2'>[[1yad]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YAD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YAD FirstGlance]. <br>
-YAD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with SO4 and 144 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1YAD OCA].
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=144:TRIS-HYDROXYMETHYL-METHYL-AMMONIUM'>144</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-==Reference==
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1yad FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yad OCA], [https://pdbe.org/1yad PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1yad RCSB], [https://www.ebi.ac.uk/pdbsum/1yad PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1yad ProSAT]</span></td></tr>
-Structural characterization of the regulatory proteins TenA and TenI from Bacillus subtilis and identification of TenA as a thiaminase II., Toms AV, Haas AL, Park JH, Begley TP, Ealick SE, Biochemistry. 2005 Feb 22;44(7):2319-29. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15709744 15709744]
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/TENI_BACSU TENI_BACSU] Reduces the stimulatory effect of the TenA transcriptional activator on production of certain extracellular enzymes. Is not an essential protein, but affects the sporulation frequency.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ya/1yad_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1yad ConSurf].
+<div style="clear:both"></div>
+__TOC__
+</StructureSection>
 [[Category: Bacillus subtilis]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Begley, T.P.]]
+[[Category: Begley TP]]
-[[Category: Ealick, S.E.]]
+[[Category: Ealick SE]]
-[[Category: Haas, A.L.]]
+[[Category: Haas AL]]
-[[Category: Park, J.H.]]
+[[Category: Park J-H]]
-[[Category: Toms, A.V.]]
+[[Category: Toms AV]]
-[[Category: 144]]
-[[Category: SO4]]
-[[Category: tim barrel]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 06:39:08 2007''

1yad

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Structure of TenI from Bacillus subtilis

Structural highlights

Function

Evolutionary Conservation

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